miRNAs from Plasma Extracellular Vesicles Are Signatory Noninvasive Prognostic Biomarkers against Atherosclerosis in LDLr -/- Mice.

Autor: Zhai KF; Department of Clinical Laboratory, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China.; State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Guangxi Normal University), Guilin 541004, China.; State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China., Duan H; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China., Shi Y; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China., Zhou YR; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China., Chen Y; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China., Zhang YS; Faculty of Pharmacy, Bengbu Medical College, Bengbu 233030, China., Gong ZP; State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Key Laboratory of Pharmaceutics, Engineering Research Center for the Development and Application of Ethnic Medicine and TCM (Ministry of Education), Guizhou Medical University, Guiyang 550004, China., Cao WG; Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, 49, Bianhe Road, Suzhou 234000, China., Wu J; Department of Clinical Laboratory, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China., Wang JJ; Department of Clinical Laboratory, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2022 Aug 17; Vol. 2022, pp. 6887192. Date of Electronic Publication: 2022 Aug 17 (Print Publication: 2022).
DOI: 10.1155/2022/6887192
Abstrakt: Circular microRNAs (miRNAs) have become central in pathophysiological conditions of atherosclerosis (AS). However, the biomarkers for diagnosis and therapeutics against AS are still unclear. The atherosclerosis models in low-density lipoprotein receptor deficiency (LDLr -/- ) mice were established with a high-fat diet (HFD). The extraction kit isolated extracellular vesicles from plasma. Total RNAs were extracted from LDLr -/- mice in plasma extracellular vesicles. Significantly varying miRNAs were detected by employing Illumina HiSeq 2000 deep sequencing technology. Target gene predictions of miRNAs were employed by related software that include RNAhybrid, TargetScan, miRanda, and PITA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) further analyzed the intersection points of predicted results. The results showed that the HFD group gradually formed atherosclerotic plaques in thoracic aorta compared with the control group. Out of 17, 8 upregulated and 9 downregulated miRNAs with a significant difference were found in the plasma extracellular vesicles that were further cross-examined by sequencing and bioinformatics analysis. Focal adhesion and Ras signaling pathway were found to be the most closely related pathways through GO and KEGG pathway analyses. The 8 most differentially expressed up- and downregulated miRNAs were further ascertained by TaqMan-based qRT-PCR. TaqMan-based qRT-PCR and in situ hybridization further validated the most differentially expressed miRNAs (miR-378d, miR-181b-5p, miR-146a-5p, miR-421-3p, miR-350-3p, and miR-184-3p) that were consistent with deep sequencing analysis suggesting a promising potential of utility to serve as diagnostic biomarkers against AS. The study gives a comprehensive profile of circular miRNAs in atherosclerosis and may pave the way for identifying biomarkers and novel targets for atherosclerosis.
Competing Interests: The authors declare that there are no conflicts of interest.
(Copyright © 2022 Ke-feng Zhai et al.)
Databáze: MEDLINE
Externí odkaz: