Biological Validation of a Chemical Effluent Decontamination System.

Autor:	Cote CK; Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Weidner JM; Medical Translational Sciences Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Klimko C; Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Piper AE; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Miller JA; Biosafety Office, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Hunter M; Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Shoe JL; Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Hoover JC; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Sauerbry BR; Logistics Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Buhr T; Naval Surface Warfare Center, Dahlgren Division, Dahlgren, VA, USA., Bozue JA; Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Harbourt DE; Biosafety Office, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA., Glass PJ; Virology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD, USA.
Jazyk:	angličtina
Zdroj:	Applied biosafety : journal of the American Biological Safety Association [Appl Biosaf] 2021 Mar 01; Vol. 26 (1), pp. 23-32. Date of Electronic Publication: 2021 Mar 19.
DOI:	10.1089/apb.21.937967
Abstrakt:	Introduction: Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. Methods: Studies were conducted in the laboratory to validate inactivation of Bacillus spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. Results: These studies demonstrated effective decontamination of >10 6 spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. Discussion: The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal. (Copyright 2021, ABSA International 2021.)
Databáze:	MEDLINE
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