Q-TOF LC-MS compounds evaluation of propolis extract derived from Malaysian stingless bees, Tetrigona apicalis , and their bioactivities in breast cancer cell, MCF7.

Autor: Mohamed WAS; Centre for Coordination of Clinical Research Network (CCRN), Block B4, Institute for Clinical Research (ICR), National Institutes of Health (NIH), Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, 40170 Shah Alam, Selangor, Malaysia.; Centre for Clinical Trial (CCT), Block B4, Institute for Clinical Research (ICR), National Institutes of Health (NIH), Jalan Setia Murni U13/52, Seksyen U13, Setia Alam, 40170 Shah Alam, Selangor, Malaysia., Ismail NZ; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia., Muhamad M; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia., Omar EA; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia., Abdul Samad N; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia., Ooi JP; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia.; Breast Cancer Translational Research Program (BCTRP@IPPT), Advanced Medical and Dental Institute (IPPT), Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia., Mohamad S; Department of Toxicology, Advanced Medical and Dental Institute (IPPT) Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia.; Breast Cancer Translational Research Program (BCTRP@IPPT), Advanced Medical and Dental Institute (IPPT), Universiti Sains Malaysia, SAINS@BERTAM, 13200 Kepala Batas, Penang, Malaysia.
Jazyk: angličtina
Zdroj: Saudi journal of biological sciences [Saudi J Biol Sci] 2022 Oct; Vol. 29 (10), pp. 103403. Date of Electronic Publication: 2022 Aug 05.
DOI: 10.1016/j.sjbs.2022.103403
Abstrakt: Propolis is known to exhibit various phytochemical compounds that aid in several biological activities. The current study investigates the phytochemical compounds of ethanolic extract of propolis of Tetrigona apicalis (EEP) using Q-TOF LC-MS, its antioxidant properties using DPPH and ABTS + radical scavenging assays, total phenolic (TPC) and flavonoid content (TFC), using Folin-Ciocalteu and Aluminium Chloride method, respectively, as well as proapoptotic effects, based on the selected IC 50 of the cytotoxic study conducted for EEP using annexin V-FITC assay. Terpene and polyphenol were among of 17 identified compounds. The EC 50 of EEP for DPPH and ABTS + was 1.78 mg/mL and 1.68 mg/mL, while the EEP exhibited TPC and TFC values of 31.99 mgGAE/g and 66.4 mgQCE/g, respectively in which the parameters were strongly correlated. The IC 50 of EEP effectively induces apoptosis in MCF7 cells. In conclusion, EEP possessed important phytochemical compounds that work excellently as antioxidants and anticancer agents.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2022 The Authors.)
Databáze: MEDLINE
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