Fabrication of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease.

Autor: Duong ND; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City, Vietnam., Nguyen-Phuoc KH; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Mai-Hoang TD; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Do KT; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City, Vietnam., Huynh TB; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Nguyen NT; Pasteur Institute in Ho Chi Minh City, Ho Chi Minh City, Vietnam., Tran TL; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam., Tran-Van H; Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, Vietnam.; Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.; Vietnam National University, Ho Chi Minh City, Vietnam.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2022 Oct; Vol. 12 (10), pp. 243. Date of Electronic Publication: 2022 Aug 24.
DOI: 10.1007/s13205-022-03311-2
Abstrakt: Acute hepatopancreatic necrosis disease (AHPND) is a contagious disease for the shrimp cultivation, thus early detection of disease is an unmet need. This present study documented for the first time a simple lateral flow immunoassay (LFIA) strip using polyclonal antibodies was created for the rapid detection both of PirA vp  and PirB vp  protein simultaneously. LFIA method based on the principle of sandwich format. The label is the colloidal gold. The polyclonal antibody was conjugated with the colloidal gold acting as biorecognition element and coated onto the conjugate pad. The rabbit anti-Pir vp , anti-PirB vp  antibodies, and goat anti-rabbit IgG antibody were separately sprayed onto a nitrocellulose membrane to form two test lines and one control line, respectively. The appearance of red bands at the control line and the test line indicated a positive result. A single coloured band at control area indicated a negative result. The limit of detection of LFIA was found to be 125 ng, which could be visually detected by naked eye within 15 min. There was no cross-reactivity observed with VP non-AHPND . Furthermore, the sensitivity and specificity of LFIA were 94.0% and 98.0%, respectively. The developed test strip could be a game changer for early and in situ diagnosis of AHPND.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest in the publication.
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Databáze: MEDLINE
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