Self-fluorescence property of octa-arginine functionalized hydroxyapatite nanoparticles aids in studying their intracellular fate in R1 ESCs.

Autor: Zantye P; Department of Biological Sciences, BITS Pilani K.K. Birla Goa Campus, Zuarinagar, Goa, 403726, India. Electronic address: p20160003@goa.bits-pilani.ac.in., Talukdar I; Department of Biological Sciences, BITS Pilani K.K. Birla Goa Campus, Zuarinagar, Goa, 403726, India. Electronic address: indranit@goa.bits-pilani.ac.in., Ramanan SR; Department of Chemical Engineering, BITS Pilani K.K. Birla Goa Campus, Zuarinagar, Goa, 403726, India. Electronic address: sutapa@goa.bits-pilani.ac.in., Kowshik M; Department of Biological Sciences, BITS Pilani K.K. Birla Goa Campus, Zuarinagar, Goa, 403726, India. Electronic address: meenal@goa.bits-pilani.ac.in.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2022 Oct 30; Vol. 627, pp. 21-29. Date of Electronic Publication: 2022 Aug 09.
DOI: 10.1016/j.bbrc.2022.08.012
Abstrakt: Deciphering the endocytosis mechanisms of nanoparticle entry in cells is crucial to understand the fate of nanoparticles and the biological activity of the transported cargo. Such studies require the use of reporter agents such as fluorescent markers. Previously, we have reported the synthesis of self-fluorescent HAp nanoparticles as efficient nucleic acid delivery agents in prokaryotic and eukaryotic cells. Here, we show the application of biocompatible self-fluorescent nano delivery vehicle based on HAp and CPP- octa-arginine as an efficient system to study the mechanisms of intracellular fate of a gene delivery agent. The pathway of octa-arginine functionalized HAp NP (R8HNP) and HAp NP uptake in R1 ESCs was elucidated using confocal microscopy with the help of endocytic inhibitors. The NPs mainly enter R1 ESCs by clathrin mediated and macropinocytosis pathways. It was established that the NPs escape endosomal degradation by proton sponge effect owing to their ability to buffer the pH and trigger osmotic rupture. The functionalization of CPP, effectively enhanced the internalization and endosomal escape in R1 ESCs. The detailed results of these studies are outlined in this manuscript.
Competing Interests: Declaration of competing interest The authors state that there are no conflicts to declare.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: