Biotransformation of (-)-Isopulegol by Rhodococcus rhodochrous .

Autor: Ivshina IB; Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia.; Department of Microbiology and Immunology, Perm State National Research University, 15 Bukirev Str., 614990 Perm, Russia., Luchnikova NA; Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences, Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences, 13 Golev Str., 614081 Perm, Russia.; Department of Microbiology and Immunology, Perm State National Research University, 15 Bukirev Str., 614990 Perm, Russia., Maltseva PY; Department of Microbiology and Immunology, Perm State National Research University, 15 Bukirev Str., 614990 Perm, Russia., Ilyina IV; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia., Volcho KP; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia., Gatilov YV; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia., Korchagina DV; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia., Kostrikina NA; Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, Russia., Sorokin VV; Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, Russia., Mulyukin AL; Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 60 let Oktyabrya, 7, bld. 2, 117312 Moscow, Russia., Salakhutdinov NF; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of the Siberian Branch of the Russian Academy of Sciences, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia.
Jazyk: angličtina
Zdroj: Pharmaceuticals (Basel, Switzerland) [Pharmaceuticals (Basel)] 2022 Aug 03; Vol. 15 (8). Date of Electronic Publication: 2022 Aug 03.
DOI: 10.3390/ph15080964
Abstrakt: The ability of actinobacteria of the genus Rhodococcus to biotransform the monoterpenoid (-)-isopulegol has been established for the first time. R . rhodochrous strain IEGM 1362 was selected as a bacterium capable of metabolizing (-)-isopulegol to form new, previously unknown, 10-hydroxy ( 2 ) and 10-carboxy ( 3 ) derivatives, which may presumably have antitumor activity and act as respiratory stimulants and cancer prevention agents. In the experiments, optimal conditions were selected to provide the maximum target catalytic activity of rhodococci. Using up-to-date (TEM, AFM-CLSM, and EDX) and traditional (cell size, roughness, and zeta potential measurements) biophysical and microbiological methods, it was shown that (-)-isopulegol and halloysite nanotubes did not negatively affect the bacterial cells. The data obtained expand our knowledge of the biocatalytic potential of rhodococci and their possible involvement in the synthesis of pharmacologically active compounds from plant derivatives.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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