| Autor: |
Rojas-Rechy MH; Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico.; Postgraduate Program in Biomedicine and Molecular Biotechnology, National Polytechnic Institute, Mexico City 11340, Mexico., Gaytán-Morales F; Bone Marrow Transplant Service, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Sánchez-Ponce Y; Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Castorena-Villa I; Bone Marrow Transplant Service, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., López-Martínez B; Sub-Direction of Diagnostic Auxiliary Services, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Parra-Ortega I; Department of Clinical Laboratory, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Escamilla-Núñez MC; National Institute of Public Health, Cuernavaca 62100, Mexico., Méndez-Tenorio A; Laboratorio de Biotecnología y Bioinformática Genómica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico., Pompa-Mera EN; Infectious and Parasitic Disease Medical Research Unit (UIMEIP), Pediatric Hospital in National Medical Center (CMN-SIGLO XXI), Mexican Institute of Social Security (IMSS), Mexico City 06720, Mexico., Martinez-Ruiz GU; División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.; Laboratorio de Investigación en Patología Experimental, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Fuentes-Pananá EM; Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico., Morales-Sánchez A; Research Unit in Virology and Cancer, Children's Hospital of Mexico Federico Gomez, Mexico City 06720, Mexico. |
| Abstrakt: |
Infections remain a major cause of morbidity and mortality among hematopoietic stem cell transplant (HSCT) recipients. Unlike Epstein-Barr Virus (EBV) and Human Cytomegalovirus (HCMV), Human Herpesvirus (HHV) 6, HHV7 and HHV8 are not routinely monitored in many centers, especially in the pediatric population of low-medium income countries. We screened EBV, HCMV, HHV6, HHV7 and HHV8 in 412 leukocytes-plasma paired samples from 40 pediatric patients assisted in a tertiary hospital in Mexico. Thirty-two underwent allo-HSCT, whereas eight received auto-HSCT. Overall viral detection frequencies in allo- and auto-HSCT were: EBV = 43.7% and 30.0%, HCMV = 5.0% and 6.7%, HHV6 = 7.9% and 20.0% and HHV7 = 9.7% and 23.3%. HHV8 was not detected in any sample. Interestingly, HHV6 and HHV7 were more frequent in auto-HSCT, and HHV6 was observed in all episodes of multiple detection in auto-HSCT patients. We found EBV DNA in plasma samples, whereas HCMV, HHV6 and HHV7 DNA were predominantly observed in leukocytes, indicative of their expansion in cellular compartments. We also found that IL-1β, IL-2, IL-6 and IL-8 were significantly increased in episodes in which multiple viruses were simultaneously detected, and samples positive for EBV DNA and graft-versus-host disease had a further increase of IL-1β and IL-8. In conclusion, the EBV, HCMV, HHV6 and HHV7 burdens were frequently detected in allo- and auto-HSCT, and their presence associated with systemic inflammation. |
