Regulation of Host Immune Response against Enterobacter cloacae Proteins via Computational mRNA Vaccine Design through Transcriptional Modification.

Autor:	Naveed M; Department of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, Pakistan., Jabeen K; Department of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, Pakistan., Naz R; Corona Intensive Care Units, District Headquarter Teaching Hospital, Dera Ghazi Khan 33000, Punjab, Pakistan., Mughal MS; Department of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, Pakistan., Rabaan AA; Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran 31311, Saudi Arabia.; College of Medicine, Alfaisal University, Riyadh 11533, Saudi Arabia.; Department of Public Health and Nutrition, The University of Haripur, Haripur 22610, Pakistan., Bakhrebah MA; Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia., Alhoshani FM; Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia., Aljeldah M; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi Arabia., Shammari BRA; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39831, Saudi Arabia., Alissa M; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia., Sabour AA; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Alaeq RA; Department of Medical Laboratories Technology, Faculty of Applied Medical Science, Taibah University, Al Madinah Al Munawarh 344, Saudi Arabia., Alshiekheid MA; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Garout M; Department of Community Medicine and Health Care for Pilgrims, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi Arabia., Almogbel MS; Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Hail, Hail 4030, Saudi Arabia., Halwani MA; Department of Medical Microbiology, Faculty of Medicine, Al Baha University, Al Baha 4781, Saudi Arabia., Turkistani SA; Department of Medical Laboratory, Fakeeh College for Medical Science, Jeddah 21134, Saudi Arabia., Ahmed N; Department of Biotechnology, Faculty of Sciences and Technology, University of Central Punjab, Lahore 54590, Pakistan.; Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.
Jazyk:	angličtina
Zdroj:	Microorganisms [Microorganisms] 2022 Aug 10; Vol. 10 (8). Date of Electronic Publication: 2022 Aug 10.
DOI:	10.3390/microorganisms10081621
Abstrakt:	Enterobacter cloacae is mainly responsible for sepsis, urethritis, and respiratory tract infections. These bacteria may affect the transcription of the host and particularly their immune system by producing changes in their epigenetics. In the present study, four proteins of Enterobacter cloacae were used to predict the epitopes for the construction of an mRNA vaccine against Enterobacter cloacae infections. In order to generate cellular and humoral responses, various immunoinformatic-based approaches were used for developing the vaccine. The molecular docking analysis was performed for predicting the interaction among the chosen epitopes and corresponding MHC alleles. The vaccine was developed by combining epitopes (thirty-three total), which include the adjuvant Toll-like receptor-4 (TLR4). The constructed vaccine was analyzed and predicted to cover 99.2% of the global population. Additionally, in silico immunological modeling of the vaccination was also carried out. When it enters the cytoplasm of the human (host), the codon is optimized to generate the translated mRNA efficiently. Moreover, the peptide structures were analyzed and docked with TLR-3 and TLR-4. A dynamic simulation predicted the stability of the binding complex. The assumed construct was considered to be a potential candidate for a vaccine against Enterobacter cloacae infections. Hence, the proposed construct is suitable for in vitro analyses to validate its effectiveness.
Databáze:	MEDLINE
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