| Autor: |
Igna V; Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 300645 Timisoara, Romania., Telea A; Center for Gene and Cellular Therapies in the Treatment of Cancer-OncoGen, Clinical Emergency County Hospital 'Pius Brinzeu' Timisoara, 300723 Timisoara, Romania., Florea T; Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 300645 Timisoara, Romania., Popp R; Faculty of Veterinary Medicine, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 300645 Timisoara, Romania., Grozea A; Faculty of Bioengineering of Animal Resources, Banat's University of Agricultural Sciences and Veterinary Medicine 'King Michael I of Romania' from Timisoara, 300645 Timisoara, Romania. |
| Jazyk: |
angličtina |
| Zdroj: |
Animals : an open access journal from MDPI [Animals (Basel)] 2022 Aug 22; Vol. 12 (16). Date of Electronic Publication: 2022 Aug 22. |
| DOI: |
10.3390/ani12162153 |
| Abstrakt: |
The alarming decline in sturgeon populations doubled by growing demands for sturgeon products, urge us to prevent the loss of these species. Fish stocking in natural habitats and developing fish farms are viable solutions, which can be successfully implemented with the help of reproductive biotechnologies. Despite the fact that semen cryopreservation is admittedly an important step for saving the Russian sturgeon, a reproducible standard method with good results has yet to be identified. Sperm quality assessment is essential for quantifying the impact of cryopreservation on spermatozoa. The purpose of our study was to provide additional information regarding semen cryopreservation and semen quality evaluation for the Russian sturgeon. Our study method is based on the use of two yolk-free extenders (with different cryoprotectants: DMSO and methanol) for freezing semen, using a simple freezing protocol. Parameters such as volume, concentration, motility, morphology and membrane integrity were evaluated. In conclusion, cryopreservation of Russian sturgeon spermatozoa using an extender containing methanol as cryoprotectant led to high egg fertilization percentages (72.67 ± 5.4%) even if the total motility values recorded for thawed semen were quite low (18-25%). Additionally, we identified two optimal stains for morphological studies and morphometry (Spermac stain kit and Trypan Blue Solution). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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