Structural and Functional Characterization of Orcokinin B-like Neuropeptides in the Cuttlefish ( Sepia officinalis ).

Autor: Endress M; Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Muséum National D'histoire Naturelle, Sorbonne Université, Université de Caen-Normandie, Université des Antilles, CNRS-8067, IRD 207, Normandy University, F-75231 Paris, France.; UNICAEN, Sorbonne Université, MNHN, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), F-14032 Caen, France., Zatylny-Gaudin C; Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Muséum National D'histoire Naturelle, Sorbonne Université, Université de Caen-Normandie, Université des Antilles, CNRS-8067, IRD 207, Normandy University, F-75231 Paris, France.; UNICAEN, Sorbonne Université, MNHN, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), F-14032 Caen, France., Leprince J; INSERM U1239, Rouen Normandy University, Neuroendocrine, Endocrine and Germinal Differentiation and Communication (NorDiC), PRIMACEN, F-76000 Rouen, France., Lefranc B; INSERM U1239, Rouen Normandy University, Neuroendocrine, Endocrine and Germinal Differentiation and Communication (NorDiC), PRIMACEN, F-76000 Rouen, France., Corre E; CNRS, Sorbonne Université, FR2424 ABiMS, Station Biologique, F-29680 Roscoff, France., Le Corguillé G; CNRS, Sorbonne Université, FR2424 ABiMS, Station Biologique, F-29680 Roscoff, France., Bernay B; Normandy University, Post Genomic Platform PROTEOGEN, SF ICORE 4206, F-14032 Caen, France., Leduc A; Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Muséum National D'histoire Naturelle, Sorbonne Université, Université de Caen-Normandie, Université des Antilles, CNRS-8067, IRD 207, Normandy University, F-75231 Paris, France.; UNICAEN, Sorbonne Université, MNHN, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), F-14032 Caen, France., Rangama J; Normandy University, CIMAP, UMP 6252 (CEA/CNRS/ENSICAEN/Normandy University), F-14032 Caen, France., Mouret L; Université Rennes, CNRS, ISCR-UMR 6226, COrInt, F-35000 Rennes, France., Lafont AG; Université Rennes, CNRS, ISCR-UMR 6226, COrInt, F-35000 Rennes, France., Bondon A; Université Rennes, CNRS, ISCR-UMR 6226, COrInt, F-35000 Rennes, France., Henry J; Biologie des Organismes et Écosystèmes Aquatiques (BOREA) Muséum National D'histoire Naturelle, Sorbonne Université, Université de Caen-Normandie, Université des Antilles, CNRS-8067, IRD 207, Normandy University, F-75231 Paris, France.; UNICAEN, Sorbonne Université, MNHN, UA, CNRS, IRD, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), F-14032 Caen, France.; Normandy University, Post Genomic Platform PROTEOGEN, SF ICORE 4206, F-14032 Caen, France.
Jazyk: angličtina
Zdroj: Marine drugs [Mar Drugs] 2022 Aug 04; Vol. 20 (8). Date of Electronic Publication: 2022 Aug 04.
DOI: 10.3390/md20080505
Abstrakt: The cuttlefish ( Sepia officinalis ) is a Cephalopod mollusk that lives in the English Channel and breeds in coastal spawning grounds in spring. A previous work showed that the control of egg-laying is monitored by different types of regulators, among which neuropeptides play a major role. They are involved in the integration of environmental cues, and participate in the transport of oocytes in the genital tract and in the secretion of capsular products. This study addresses a family of neuropeptides recently identified and suspected to be involved in the control of the reproduction processes. Detected by mass spectrometry and immunocytochemistry in the nerve endings of the accessory sex glands of the females and ovary, these neuropeptides are also identified in the hemolymph of egg-laying females demonstrating that they also have a hormone-like role. Released in the hemolymph by the sub-esophageal mass, a region that innervates the genital tract and the neurohemal area of the vena cava, in in vitro conditions these neuropeptides modulated oocyte transport and capsular secretion. Finally, in silico analyses indicated that these neuropeptides, initially called FLGamide, had extensive structural homology with orcokinin B, which motivated their name change.
Databáze: MEDLINE
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