| Autor: |
Anwer MA; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Niwas R; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Ranjan T; Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Mandal SS; Department of Plant Breeding and Genetics, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Ansar M; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Srivastava JN; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Kumar J; Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Jain K; Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Kumari N; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India., Bharti A; Department of Plant Pathology, Bihar Agricultural University, Sabour 813210, Bhagalpur, India. |
| Abstrakt: |
Maize is considered the third most important cereal crop in Asia after rice and wheat. Many diseases affect this crop due to the cultivation of various hybrids. This research aimed to characterize the causative agent of northern corn leaf blight disease in Bihar, India, caused by Exserohilum turcicum (Passerini) Leonard and Suggs. Leaf samples were collected from infected fields in five maize growing districts of Bihar in 2020-2022. A total of 45 fungal isolates from 135 samples were examined for cultural, morphological, and molecular characteristics and were identified as E. turcicum . The isolates were grouped into four groups based on colony color, i.e., olivaceous brown, blackish brown, whitish black, and grayish, and into two groups based on regular and irregular margins. The conidial shapes were observed to be elongated and spindle-shaped with protruding hilum, with conidial septa ranging from 2-12. Similarly, conidial length varied from 52.94 μm to 144.12 μm. β -tubulin gene sequences analysis made it possible to verify the identities of fungal strains and the phylogenetic relationships of all isolates, which were clustered in the same clade. The β -tubulin gene sequences of all the isolates showed a high level of similarity (100%) with reference isolates from GenBank accession numbers KU670342.1, KU670344.1, KU670343.1, KU670341.1, and KU670340.1. The findings of this study will serve as a baseline for future studies and will help to minimize yield losses. |
