Induced variations of ethyl methane sulfonate mutagenized cowpea ( Vigna unguiculata L. walp) plants.

Autor: Opoku Gyamfi M; West Africa Centre for Crop Improvement, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.; Department of Crop Science, School of Agriculture, University of Ghana, Accra, Ghana., Eleblu JSY; West Africa Centre for Crop Improvement, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.; Biotechnology Centre, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Sarfoa LG; Department of Crop Science, School of Agriculture, University of Ghana, Accra, Ghana.; Biotechnology Centre, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana., Asante IK; West Africa Centre for Crop Improvement, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.; Department of Plant and Environmental Biology, University of Ghana, Accra, Ghana., Opoku-Agyemang F; West Africa Centre for Crop Improvement, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.; Department of Crop Science, School of Agriculture, University of Ghana, Accra, Ghana., Danquah EY; West Africa Centre for Crop Improvement, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.; Department of Crop Science, School of Agriculture, University of Ghana, Accra, Ghana.; Biotechnology Centre, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana.
Jazyk: angličtina
Zdroj: Frontiers in plant science [Front Plant Sci] 2022 Aug 05; Vol. 13, pp. 952247. Date of Electronic Publication: 2022 Aug 05 (Print Publication: 2022).
DOI: 10.3389/fpls.2022.952247
Abstrakt: Unique variants are desired in the development of genetically improved crops to meet farmer and market needs hence ethyl methane sulfonate (EMS) was used to induce genetic variability in cowpea ( Vigna unguiculata cv. Asontem ). The main objective of this research was to characterize induced variations in EMS chemically mutagenized population of cowpea ( Vigna unguiculata L. Walp Var. Asontem) in the M 1 and M 2 generations. The optimum concentration (LD50) of EMS for generating the mutagenized population was determined by treating seeds with different concentrations of EMS (0.0, 0.2, 0.4, 0.6, and 0.8% v/v) and observing the germination count after 5 days of planting the seeds in Petri dishes. Three thousand cowpea seeds were treated with the 0.4% EMS to generate the M 1 and M 2 populations that were evaluated for agronomic and morphological traits with untreated seeds serving as control. Data analysis involved distribution of qualitative and quantitative traits. Germination was significantly reduced in the mutagenized population (17.8%) and compared with that of the wild type (61.6%). Percentage survival was significantly higher in wild type (98.38%) as compared with the M 1 population (78.46%). Percentage germination in the M 2 population (74.03%) was lower than the wild type (80%). A wide spectrum of agro-morphological abnormalities was observed in the M 2 population. Wide variations and uniquely different phenotypic classes were observed in leaf color, leaf shape, growth habit, plant pigmentation, twining tendency, pod curvature, seed shape, and seed coat color. M 2 individuals were widely distributed for days to flowering, number of pods per plant, number of seeds per pod, number of locules per pods, percentage seed set, pod length and number of seeds per plant. In conclusion, the EMS mutagenesis was effective in inducing the unique variations that will be useful for breeding and development of new farmer preferred varieties.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Opoku Gyamfi, Eleblu, Sarfoa, Asante, Opoku-Agyemang and Danquah.)
Databáze: MEDLINE
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