Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

Autor: Rossatti P; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland., Redpath GMI; EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, Sydney, Australia., Ziegler L; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland.; Department of Biology, University of Konstanz, Constance, Germany., Samson GPB; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland., Clamagirand CD; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland., Legler DF; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland.; Department of Biology, University of Konstanz, Constance, Germany., Rossy J; Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280, Kreuzlingen, Switzerland. jeremie.rossy@bitg.ch.; Department of Biology, University of Konstanz, Constance, Germany. jeremie.rossy@bitg.ch.
Jazyk: angličtina
Zdroj: BMC biology [BMC Biol] 2022 Aug 24; Vol. 20 (1), pp. 189. Date of Electronic Publication: 2022 Aug 24.
DOI: 10.1186/s12915-022-01386-0
Abstrakt: Background: T cell activation leads to increased expression of the receptor for the iron transporter transferrin (TfR) to provide iron required for the cell differentiation and clonal expansion that takes place during the days after encounter with a cognate antigen. However, T cells mobilise TfR to their surface within minutes after activation, although the reason and mechanism driving this process remain unclear.
Results: Here we show that T cells transiently increase endocytic uptake and recycling of TfR upon activation, thereby boosting their capacity to import iron. We demonstrate that increased TfR recycling is powered by a fast endocytic sorting pathway relying on the membrane proteins flotillins, Rab5- and Rab11a-positive endosomes. Our data further reveal that iron import is required for a non-canonical signalling pathway involving the kinases Zap70 and PAK, which controls adhesion of the integrin LFA-1 and eventually leads to conjugation with antigen-presenting cells.
Conclusions: Altogether, our data suggest that T cells boost their iron importing capacity immediately upon activation to promote adhesion to antigen-presenting cells.
(© 2022. The Author(s).)
Databáze: MEDLINE
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