Immune response induced in rodents by anti-CoVid19 plasmid DNA vaccine via pyro-drive jet injector inoculation.

Autor:	Nishikawa T; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Osaka, Japan., Chang CY; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Osaka, Japan., Tai JA; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Osaka, Japan., Hayashi H; Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan., Sun J; Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan., Torii S; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Ono C; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Matsuura Y; Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan., Ide R; FunPep Co., Ltd, Tokyo, Japan., Mineno J; Takara Bio Inc, Shiga, Japan., Sasai M; Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Yamamoto M; Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan., Nakagami H; Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan., Yamashita K; Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Osaka, Japan.; Medical Device Research, Industry Business Unit, Daicel Corporation, Osaka, Japan.
Jazyk:	angličtina
Zdroj:	Immunological medicine [Immunol Med] 2022 Dec; Vol. 45 (4), pp. 251-264. Date of Electronic Publication: 2022 Aug 24.
DOI:	10.1080/25785826.2022.2111905
Abstrakt:	There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through the development of efficient and safe vaccination methods. Over the short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport and storage. pVAX1-SARS-CoV2-co was designed for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led to immunoreactions against the S protein, an anti-receptor-binding-domain, and a neutralizing action of the pVAX1-SARS-CoV2-co, as previously confirmed. To promote the efficacy of the pVAX1-SARS-CoV2-co vaccine a pyro-drive jet injector (PJI) was used. An intradermally adjusted PJI demonstrated that the pVAX1-SARS-CoV2-co vaccine injection caused a high production of anti-S protein antibodies, triggered immunoreactions, and neutralized the actions against SARS-CoV-2. A high-dose pVAX1-SARS-CoV2-co intradermal injection using PJI did not cause any serious disorders in the rat model. A viral challenge confirmed that intradermally immunized mice were potently protected from COVID-19. A pVAX1-SARS-CoV2-co intradermal injection using PJI is a safe and promising vaccination method for overcoming the COVID-19 pandemic.
Databáze:	MEDLINE
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