Evaluation of the Boson rapid Ag test vs RT-PCR for use as a self-testing platform.

Autor: Leventopoulos M; Department of Cellular Biology and Immunology, Locus Medicus S.A., Athens, Greece. Electronic address: m_leventopoulos@yahoo.com., Michou V; Department of Molecular Pathology and Genetics, Locus Medicus S.A., Athens, Greece., Papadimitropoulos M; Department of Molecular Pathology and Genetics, Locus Medicus S.A., Athens, Greece., Vourva E; Department of Molecular Pathology and Genetics, Locus Medicus S.A., Athens, Greece., Manias NG; Department of Statistics, Locus Medicus S.A., Athens, Greece., Kavvadas HP; Department of Cellular Biology and Immunology, Locus Medicus S.A., Athens, Greece., Nikolopoulos D; Department of Cellular Biology and Immunology, Locus Medicus S.A., Athens, Greece., Tsilivakos V; Department of Cellular Biology and Immunology, Locus Medicus S.A., Athens, Greece., Georgoulias G; Department of Biopathology, Locus Medicus S.A., Athens, Greece.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2022 Nov; Vol. 104 (3), pp. 115786. Date of Electronic Publication: 2022 Jul 29.
DOI: 10.1016/j.diagmicrobio.2022.115786
Abstrakt: The gold standard test available for detecting COVID-19 patients is Real Time RT-PCR. However, this method is expensive, needing special equipment and skilled laboratory staff. Recently, less expensive antigen tests have become available, that could easily and rapidly identify new COVID-19 cases. Our objective was to evaluate the Boson Rapid Antigen Test Card versus the RT-rtPCR, using samples taken both by laymen (self-testing) and professionals. The sensitivity, specificity and accuracy rates were, 98.18%, 100.00%, and 99.28%, respectively. The positive and negative predictive values were 100.00% and 98.82%, respectively. The detection rate for asymptomatic patients was 90.48%, and detection rate for Ct values ≥30 was 91.67%. Our results indicate a high coincidence rate between the Boson and the referencing RT-rtPCR method, meeting the performance standards recommended by the WHO. Therefore, this test could facilitate a fast self-testing screening method, for the detection of infected individuals.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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