| Autor: |
Eder S; Fraunhofer Institute for Silicate Research, ISC, 97082 Würzburg, Germany., Hollmann C; Institute for Virology and Immunobiology, University of Würzburg, 97078 Würzburg, Germany., Mandasari P; Institute for Virology and Immunobiology, University of Würzburg, 97078 Würzburg, Germany., Wittmann P; Fraunhofer Institute for Silicate Research, ISC, 97082 Würzburg, Germany.; Institute for Virology and Immunobiology, University of Würzburg, 97078 Würzburg, Germany., Schumacher F; Institute of Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany., Kleuser B; Institute of Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany., Fink J; Institute of Organic Chemistry, University of Würzburg, 97074 Würzburg, Germany., Seibel J; Institute of Organic Chemistry, University of Würzburg, 97074 Würzburg, Germany., Schneider-Schaulies J; Institute for Virology and Immunobiology, University of Würzburg, 97078 Würzburg, Germany., Stigloher C; Imaging Core Facility of the Biocenter, University of Würzburg, 97074 Würzburg, Germany., Beyersdorf N; Institute for Virology and Immunobiology, University of Würzburg, 97078 Würzburg, Germany., Dembski S; Fraunhofer Institute for Silicate Research, ISC, 97082 Würzburg, Germany.; Department Tissue Engineering and Regenerative Medicine, University Hospital, 97070 Würzburg, Germany. |
| Abstrakt: |
A fine balance of regulatory (T reg ) and conventional CD4 + T cells (T conv ) is required to prevent harmful immune responses, while at the same time ensuring the development of protective immunity against pathogens. As for many cellular processes, sphingolipid metabolism also crucially modulates the T reg /T conv balance. However, our understanding of how sphingolipid metabolism is involved in T cell biology is still evolving and a better characterization of the tools at hand is required to advance the field. Therefore, we established a reductionist liposomal membrane model system to imitate the plasma membrane of mouse T reg and T conv with regards to their ceramide content. We found that the capacity of membranes to incorporate externally added azide-functionalized ceramide positively correlated with the ceramide content of the liposomes. Moreover, we studied the impact of the different liposomal preparations on primary mouse splenocytes in vitro. The addition of liposomes to resting, but not activated, splenocytes maintained viability with liposomes containing high amounts of C 16 -ceramide being most efficient. Our data thus suggest that differences in ceramide post-incorporation into T reg and T conv reflect differences in the ceramide content of cellular membranes. |
