| Autor: |
Saleh KK; Department of Biology, Faculty of Science, Firat University, Elazığ, Turkey; Department of Community Health, Koya Technical Institute, Erbil Polytechnic University, Erbil, Iraq., Kirbag S; Department of Biology, Faculty of Science, Firat University, Elazig, Turkey., Dalkılıç S; Department of Biology, Faculty of Science, Firat University, Elazığ, Turkey. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of medicinal mushrooms [Int J Med Mushrooms] 2022; Vol. 24 (8), pp. 61-70. |
| DOI: |
10.1615/IntJMedMushrooms.2022044383 |
| Abstrakt: |
Terfezia is an edible seasonal ectomycorrhizal fungus that has long been recognized as a delicacy in several regions of the world. Herein, aqueous extracts from three significant Terfezia species (T claveryi, T. boudieri, and T. olbiensis) were investigated for their cytotoxic and apoptosis-induction effects on PANC-1 cells, a pancreatic cancer cell line. The MTT assay was applied to evaluate cytotoxicity. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to investigate genes associated with apoptosis, included four target genes (BAX, BCL2, CDKN1A, and TP53) and one reference gene (GAPDH). The aqueous extracts of T. claveryi, T. boudieri, and T. olbiensis exerted strong, dose-dependent inhibition of PANC-1 cell growth. Based on qRT-PCR, each extract reduced the progress of PANC-1 cells by inducing apoptosis, denoted by the upregulation of the proapoptotic genes BAX, CDKN1A, and TP53 and downregulation of the antiapoptotic gene BCL2. Ultimately, Terfezia desert truffles can be considered a functional and therapeutic food. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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