Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva.

Autor:	Moreira G; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC, United States.; Global Alliance for Rapid Diagnostics, Michigan State University, Cambridge, MI, United States., Casso-Hartmann L; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC, United States., Datta SPA; Medical Device (MDPnP) Interoperability and Cybersecurity Labs, Biomedical Engineering Program, Department of Anesthesiology, Massachusetts General Hospital, Harvard Medical School, Cambridge, MA, United States.; MIT Auto-ID Labs, Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States., Dean D; Center for Innovative Medical Devices and Sensors (REDDI Lab), Clemson University, Clemson, SC, United States.; Department of Bioengineering, Clemson University, Clemson, SC, United States., McLamore E; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC, United States.; Global Alliance for Rapid Diagnostics, Michigan State University, Cambridge, MI, United States.; Department of Agricultural Sciences, Clemson University, Clemson, SC, United States., Vanegas D; Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, SC, United States.; Global Alliance for Rapid Diagnostics, Michigan State University, Cambridge, MI, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in sensors [Front Sens (Lausanne)] 2022; Vol. 3. Date of Electronic Publication: 2022 Jul 13.
DOI:	10.3389/fsens.2022.917380
Abstrakt:	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the novel coronavirus responsible for COVID-19. Infection in humans requires angiotensin-converting enzyme II (hACE2) as the point of entry for SARS-CoV-2. PCR testing is generally definitive but expensive, although it is highly sensitive and accurate. Biosensor-based monitoring could be a low-cost, accurate, and non-invasive approach to improve testing capacity. We develop a capacitive hACE2 biosensor for intact SARS-CoV-2 detection in saliva. Laser-induced graphene (LIG) electrodes were modified with platinum nanoparticles. The quality control of LIG electrodes was performed using cyclic voltammetry. Truncated hACE2 was used as a biorecognition element and attached to the electrode surface by streptavidin-biotin coupling. Biolayer interferometry was used for qualitative interaction screening of hACE2 with UV-attenuated virions. Electrochemical impedance spectroscopy (EIS) was used for signal transduction. Truncated hACE2 binds wild-type SARS-CoV-2 and its variants with greater avidity than human coronavirus (common cold virus). The limit of detection (LoD) is estimated to be 2,960 copies/ml. The detection process usually takes less than 30 min. The strength of these features makes the hACE2 biosensor a potentially low-cost approach for screening SARS-CoV-2 in non-clinical settings with high demand for rapid testing (for example, schools and airports). Competing Interests: Conflict of Interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu