High-content analysis of neuronal morphology in human iPSC-derived neurons.

Autor:	Lickfett S; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Department of Anatomy II, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Menacho C; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Zink A; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany., Telugu NS; Max Delbrück Center for Molecular Medicine (MDC) and Berlin Institute of Health (BIH), 13125 Berlin, Germany., Beller M; Institute for Mathematical Modeling of Biological Systems, Heinrich Heine University, 40225 Düsseldorf, Germany., Diecke S; Max Delbrück Center for Molecular Medicine (MDC) and Berlin Institute of Health (BIH), 13125 Berlin, Germany., Cambridge S; Department of Anatomy II, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Dr. Senckenbergische Anatomie, Goethe-Universität Frankfurt, 60590 Frankfurt am Main, Germany., Prigione A; Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany. Electronic address: alessandro.prigione@hhu.de.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 Aug 06; Vol. 3 (3), pp. 101567. Date of Electronic Publication: 2022 Aug 06 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101567
Abstrakt:	We present a high-content analysis (HCA) protocol for monitoring the outgrowth capacity of human neurons derived from induced pluripotent stem cells (iPSCs). We describe steps to perform HCA imaging, followed by quantifying the morphology of dendrites and axons within a high-throughput system to evaluate neurons obtained through various differentiation approaches. This protocol can be used to screen for modulators of neuronal morphogenesis or neurotoxicity. The approach can be applied to patient-derived iPSCs to identify patient-specific defects and possible therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Zink et al. (2020) and Inak et al. (2021). The protocol can be used in combination with Zink et al. (2022). Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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