Bidirectional modulation of evoked synaptic transmission by pulsed infrared light.

Autor: Zhu X; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA.; Photonics Center, Boston University, 8 Saint Mary's Street, Boston, MA, 02215, USA.; Neurophotonics Center, Boston University, 24 Cummington Mall, Boston, MA, 02215, USA., Lin JW; Department of Biology, Boston University, 5 Cummington Mall, Boston, MA, 02215, USA., Sander MY; Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA. msander@bu.edu.; Photonics Center, Boston University, 8 Saint Mary's Street, Boston, MA, 02215, USA. msander@bu.edu.; Neurophotonics Center, Boston University, 24 Cummington Mall, Boston, MA, 02215, USA. msander@bu.edu.; Department of Electrical and Computer Engineering, Boston University, 8 Saint Mary's Street, Boston, MA, 02215, USA. msander@bu.edu.; Division of Materials Science and Engineering, Boston University, 15 Saint Mary's Street, Brookline, MA, 02446, USA. msander@bu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Aug 20; Vol. 12 (1), pp. 14196. Date of Electronic Publication: 2022 Aug 20.
DOI: 10.1038/s41598-022-18139-2
Abstrakt: Infrared (IR) neuromodulation (INM) has been demonstrated as a novel modulation modality of neuronal excitability. However, the effects of pulsed IR light on synaptic transmission have not been investigated systematically. In this report, the IR light (2 μm) is used to directly modulate evoked synaptic transmission at the crayfish opener neuromuscular junction. The extracellularly recorded terminal action potentials (tAPs) and evoked excitatory postsynaptic currents (EPSCs) modulated by localized IR light illumination (500 ms, 3-13 mW) aimed at the synapses are analyzed. The impact of a single IR light pulse on the presynaptic Ca 2+ influx is monitored with Ca 2+ indicators. The EPSC amplitude is enhanced, and its rising phase is accelerated under relatively low IR light power levels and localized temperature rises. Increasing the IR light power reversibly suppresses and eventually blocks the EPSCs. Meanwhile, the synaptic delay, tAP amplitude, and presynaptic Ca 2+ influx decrease monotonously with higher IR light power. It is demonstrated for the first time that IR light illumination has bidirectional effects on evoked synaptic transmission. These results highlight the efficacy and flexibility of using pulsed IR light to directly control synaptic transmission and advance our understanding of INM of neural networks.
(© 2022. The Author(s).)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje