The influence of violet LED application time on the esthetic efficacy and cytotoxicity of a 35% H 2 O 2 bleaching gel.

Autor: Ribeiro RAO; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Peruchi V; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Fernandes LO; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Anselmi C; Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Soares IPM; Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Hebling J; Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil., Costa CAS; Department of Physiology and Pathology, School of Dentistry, São Paulo State University (UNESP), Araraquara, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil. Electronic address: cas.costa@unesp.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2022 Dec; Vol. 40, pp. 103069. Date of Electronic Publication: 2022 Aug 17.
DOI: 10.1016/j.pdpdt.2022.103069
Abstrakt: Objective: To assess the potential influence of violet LED (V-LED) application time on the esthetic efficacy and cytotoxicity of a 35% H 2 O 2 bleaching gel.
Methodology: Stained and standardized enamel/dentin discs were subjected to one in-office tooth bleaching session (45 min), and the gel was either irradiated or not with V-LED. Thus, the following groups were established (n = 8): G1: No treatment (negative control, NC); G2: 35% H 2 O 2 (positive control, PC); G3: 35%H 2 O 2  + V-LED/15 min; G4: 35%H 2 O 2  + V-LED/30 min; G5: 35%H 2 O 2  + V-LED/45 min. First, esthetic efficacy was assessed (ΔE 00 and ΔWI). Discs assembled in artificial pulp chambers were subjected to the same bleaching treatments. Then, the extracts (culture medium + diffused bleaching gel components) were collected and applied to MDPC-23 pulp cells, which were analyzed for viability (Live/Dead, MTT) and oxidative stress (OxS). The amount of H 2 O 2 in the extracts was also determined (leuco crystal-violet/peroxidase). The data were subjected to ANOVA/Tukey at a 5% significance level.
Results: Although esthetic efficacy did not differ among the irradiated groups (G3, G4, and G5) (p > 0.05), their results were higher than in G2 (PC; p < 0.05). In the irradiated groups, the cell viability and OxS as well as the amount of H 2 O 2 in the extracts were statistically similar to G2 (PC), regardless of irradiation time (p > 0.05).
Conclusion: Although V-LED improves the esthetic outcome of in-office tooth bleaching, increasing irradiation time does not effect the color changes and cytotoxicity of this professional therapy.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: