The effect of hypergravity, hyperbaric pressure, and hypoxia on osteogenic differentiation of adipose stem cells.

Autor: Lingens LF; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: llingens@ukaachen.de., Ruhl T; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: truhl@ukaachen.de., Beier JP; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: jbeier@ukaachen.de., Mende W; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: mende_wolfgang@web.de., Freund G; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: gfreund@ukaachen.de., Götzl R; Department of Plastic Surgery, Hand Surgery-Burn Center, University Hospital RWTH Aachen, Pauwelsstraße 30, Aachen 52074, Germany. Electronic address: rgoetzl@ukaachen.de.
Jazyk: angličtina
Zdroj: Tissue & cell [Tissue Cell] 2022 Oct; Vol. 78, pp. 101886. Date of Electronic Publication: 2022 Aug 03.
DOI: 10.1016/j.tice.2022.101886
Abstrakt: Human adipose stem cells (ASCs) hold great potential for regenerative medicine approaches, including osteogenic regeneration of bone defects, that fail to heal autonomously. Osteogenic differentiation of stem cells is dependent on the stimulation of biophysical factors. In the present study, the effects of hypergravity, hypoxia, and hyperbaric treatment were investigated on adipose stem cell (ASC) metabolic activity, quantified by PrestoBlue conversion, and cell numbers, evaluated by crystal violet staining. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity and cresolphthalein staining of calcium deposition. Differentiation was performed for 12 days, which was accompanied by periodical stimulation. Increasing gravity forces up to 50x g did not affect ASC viability, but it enhanced osteogenic markers with a strongest effect between 20 and 30x g. Hyperbaric stimulation at 3 bar decreased ASC cell numbers but increased ALP activity and calcium deposition. Hypoxia at 8 % atmospheric oxygen did not affect ASC proliferation, while cell numbers were reduced at 3 % oxygen. Furthermore, hypoxic conditions produced opposing results on osteogenic markers, as ALP activity increased whereas cresolphthalein staining decreased upon stimulation. These data demonstrated that intermittent short duration of basal physical or chemical impulses interfere with the osteogenic differentiation of ASCs. Our findings could be of specific relevance in ASC based therapies for regenerative medicine and bone tissue engineering approaches.
Competing Interests: Declarations of interest No potential conflicts of interest, financial or otherwise are declared by the authors.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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