Detection of NDM Variants ( bla NDM-1 , bla NDM-2 , bla NDM-3 ) from Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae : First Report from Nepal.
| Autor: | Thapa A; Department of Microbiology, Golden Gate International College, Kathmandu, Nepal., Upreti MK; Department of Microbiology, Golden Gate International College, Kathmandu, Nepal., Bimali NK; Department of Microbiology, Golden Gate International College, Kathmandu, Nepal., Shrestha B; Kathmandu Model Hospital, Kathmandu, Nepal., Sah AK; Annapurna Neurological Institute and Allied Sciences, Kathmandu, Nepal., Nepal K; Department of Microbiology, Golden Gate International College, Kathmandu, Nepal., Dhungel B; Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal., Adhikari S; Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal., Adhikari N; Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal., Lekhak B; Department of Microbiology, Golden Gate International College, Kathmandu, Nepal.; Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal., Rijal KR; Central Department of Microbiology, Tribhuvan University, Kathmandu, Nepal. |
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| Jazyk: | angličtina |
| Zdroj: | Infection and drug resistance [Infect Drug Resist] 2022 Aug 11; Vol. 15, pp. 4419-4434. Date of Electronic Publication: 2022 Aug 11 (Print Publication: 2022). |
| DOI: | 10.2147/IDR.S369934 |
| Abstrakt: | Background: Increasing burden of carbapenem resistance among Enterobacterales is attributable to their ability to produce carbapenemase enzymes like metallo-beta-lactamase (MBL), Klebsiella pneumoniae carbapenemase (KPC), and OXA-type. This study aimed to determine the prevalence of carbapenemases and MBL genes (( bla Methods: A total of 2474 urine samples collected during the study period (July-December 2017) were processed at the microbiology laboratory of Kathmandu Model Hospital, Kathmandu. Isolates of E. coli and K. pneumoniae were processed for antimicrobial susceptibility testing (AST) by disc diffusion method. Carbapenem-resistant isolates were subjected to Modified Hodge Test (MHT) for phenotypic confirmation, and inhibitor-based combined disc tests for the differentiation of carbapenemase (MBL and KPC). MBL-producing isolates were screened for NDM genes by polymerase chain reaction (PCR). Results: Of the total urine samples processed, 19.5% (483/2474) showed the bacterial growth. E. coli (72.6%; 351/483) was the predominant isolate followed by K. pneumoniae (12.6%; 61/483). In AST, 4.4% (18/412) isolates of E. coli (15/351) and K. pneumonia (3/61) showed resistance towards carbapenems, while 1.7% (7/412) of the isolates was confirmed as carbapenem-resistant in MHT. In this study, all (3/3) the isolates of K. pneumoniae were KPC-producers, whereas 66.7% (10/15), 20% (3/15) and 13.3% (2/15) of the E. coli isolates were MBL, KPC and MBL/KPC (both)-producers, respectively. In PCR assay, 80% (8/10), 90% (9/10) and 100% (10/10) of the isolates were positive for bla Conclusion: Presence of NDM genes among carbapenemase-producing isolates is indicative of potential spread of drug-resistant variants. This study recommends the implementation of molecular diagnostic facilities in clinical settings for proper infection control, which can optimize the treatment therapies, and curb the emergence and spread of drug-resistant pathogens. Competing Interests: The authors report no conflicts of interest in relation to this work. (© 2022 Thapa et al.) |
| Databáze: | MEDLINE |
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