Cadmium promotes the absorption of ammonium in hyperaccumulator Solanum nigrum L. mediated by ammonium transporters and aquaporins.

Autor: Zhang LD; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Song LY; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Dai MJ; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Guo ZJ; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Wei MY; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Li J; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Xu CQ; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Zhu XY; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China., Zheng HL; Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, 361005, PR China. Electronic address: zhenghl@xmu.edu.cn.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2022 Nov; Vol. 307 (Pt 3), pp. 136031. Date of Electronic Publication: 2022 Aug 15.
DOI: 10.1016/j.chemosphere.2022.136031
Abstrakt: Cadmium (Cd) is a toxic heavy metal affecting the normal growth of plants. Nitrate (NO 3 - ) and ammonium (NH 4 + ) are the primary forms of inorganic nitrogen (N) absorbed by plants. However, the mechanism of N absorption and regulation under Cd stress remains unclear. This study found that: (1) Cd treatment affected the biomass, root length, and Cd 2+ flux in Solanum nigrum seedling roots. Specifically, 50 μM Cd significantly inhibited NO 3 - influx while increased NH 4 + influx compared with 0 and 5 μM Cd treatments measured by non-invasive micro-test technology. (2) qRT-PCR analysis showed that 50 μM Cd inhibited the expressions of nitrate transporter genes, SnNRT2;4 and SnNRT2;4-like, increased the expressions of ammonium transporter genes, SnAMT1;2 and SnAMT1;3, in the roots. (3) Under NH 4 + supply, 50 μM Cd significantly induced the expressions of the aquaporin genes, SnPIP1;5, SnPIP2;7, and SnTIP2;1. Our results showed that 50 μM Cd stress promoted NH 4 + absorption by up-regulating the gene expressions of NH 4 + transporter and aquaporins, suggesting that high Cd stress can affect the preference of N nutrition in S. nigrum.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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