RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination.

Autor:	Brault JB; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Bardin S; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Lampic M; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Carpentieri JA; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Coquand L; Institut Curie, PSL Research University, CNRS UMR144, Paris, France.; Sorbonne University, Paris, France., Penisson M; Sorbonne University, Paris, France.; INSERM UMR-S 1270, Paris, France.; Institut du Fer à Moulin, Paris, France., Lachuer H; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Victoria GS; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Baloul S; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., El Marjou F; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Boncompain G; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Miserey-Lenkei S; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Belvindrah R; Sorbonne University, Paris, France.; INSERM UMR-S 1270, Paris, France.; Institut du Fer à Moulin, Paris, France., Fraisier V; UMR 144-Cell and Tissue Imaging Facility (PICT-IBiSA), CNRS-Institut Curie, Paris, France., Francis F; Sorbonne University, Paris, France.; INSERM UMR-S 1270, Paris, France.; Institut du Fer à Moulin, Paris, France., Perez F; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Goud B; Institut Curie, PSL Research University, CNRS UMR144, Paris, France., Baffet AD; Institut Curie, PSL Research University, CNRS UMR144, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.
Jazyk:	angličtina
Zdroj:	EMBO reports [EMBO Rep] 2022 Oct 06; Vol. 23 (10), pp. e54605. Date of Electronic Publication: 2022 Aug 18.
DOI:	10.15252/embr.202254605
Abstrakt:	Radial glial (RG) cells are the neural stem cells of the developing neocortex. Apical RG (aRG) cells can delaminate to generate basal RG (bRG) cells, a cell type associated with human brain expansion. Here, we report that aRG delamination is regulated by the post-Golgi secretory pathway. Using in situ subcellular live imaging, we show that post-Golgi transport of RAB6+ vesicles occurs toward the minus ends of microtubules and depends on dynein. We demonstrate that the apical determinant Crumbs3 (CRB3) is also transported by dynein. Double knockout of RAB6A/A' and RAB6B impairs apical localization of CRB3 and induces a retraction of aRG cell apical process, leading to delamination and ectopic division. These defects are phenocopied by knockout of the dynein activator LIS1. Overall, our results identify a RAB6-dynein-LIS1 complex for Golgi to apical surface transport in aRG cells, and highlights the role of this pathway in the maintenance of neuroepithelial integrity. (© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.)
Databáze:	MEDLINE
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