Clonal serotype 1c multidrug-resistant Shigella flexneri detected in multiple institutions by sentinel-site sequencing.

Autor:	Ko KKK; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Department of Molecular Pathology, Singapore General Hospital, Singapore, Singapore.; Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore.; Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Chu JJK; Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore., Lim KM; Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore., Yingtaweesittikul H; Advanced Research Center for Computational Simulation, Chiang Mai University, Chiang Mai, Thailand., Huang W; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Tan SYL; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Goh KCM; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Tan SH; Department of Laboratory Medicine, Changi General Hospital, Singapore, Singapore., Ng TY; Department of Pathology, Sengkang General Hospital, Singapore, Singapore., Maiwald M; Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore.; Department of Pathology and Laboratory Medicine, KK Women's and Children's Hospital, Singapore, Singapore., Chia JWZ; Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore., Cao DY; Department of Microbiology, Singapore General Hospital, Singapore, Singapore., Tan YE; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Sim JHC; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Koh TH; Department of Microbiology, Singapore General Hospital, Singapore, Singapore.; Duke-NUS Medical School, Singapore, Singapore., Nagarajan N; Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore., Suphavilai C; Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore.
Jazyk:	angličtina
Zdroj:	Frontiers in medicine [Front Med (Lausanne)] 2022 Aug 01; Vol. 9, pp. 964640. Date of Electronic Publication: 2022 Aug 01 (Print Publication: 2022).
DOI:	10.3389/fmed.2022.964640
Abstrakt:	Shigella flexneri is a major diarrhoeal pathogen, and the emergence of multidrug-resistant S. flexneri is of public health concern. We report the detection of a clonal cluster of multidrug-resistant serotype 1c (7a) S. flexneri in Singapore in April 2022. Long-read whole-genome sequence analysis found five S. flexneri isolates to be clonal and harboring the extended-spectrum β-lactamases bla CTX-M-15 and bla TEM-1 . The isolates were phenotypically resistant to ceftriaxone and had intermediate susceptibility to ciprofloxacin. The S. flexneri clonal cluster was first detected in a tertiary hospital diagnostic laboratory (sentinel-site), to which the S. flexneri isolates were sent from other hospitals for routine serogrouping. Long-read whole-genome sequence analysis was performed in the sentinel-site near real-time in view of the unusually high number of S. flexneri isolates received within a short time frame. This study demonstrates that near real-time sentinel-site sequence-based surveillance of convenience samples can detect possible clonal outbreak clusters and may provide alerts useful for public health mitigations at the earliest possible opportunity. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Ko, Chu, Lim, Yingtaweesittikul, Huang, Tan, Goh, Tan, Ng, Maiwald, Chia, Cao, Tan, Sim, Koh, Nagarajan and Suphavilai.)
Databáze:	MEDLINE
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