| Autor: |
Meesaragandla B; Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Straße 4, 17489 Greifswald, Germany.; ZIK HIKE─Zentrum für Innovationskompetenz 'Humorale Immunreaktionen bei kardiovaskulären Erkrankungen', Fleischmannstraße 42, 17489 Greifswald, Germany., Hayet S; Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel.; The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel., Fine T; Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel.; The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel., Janke U; Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Straße 4, 17489 Greifswald, Germany.; ZIK HIKE─Zentrum für Innovationskompetenz 'Humorale Immunreaktionen bei kardiovaskulären Erkrankungen', Fleischmannstraße 42, 17489 Greifswald, Germany., Chai L; Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel.; The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, 91904 Jerusalem, Israel., Delcea M; Institute of Biochemistry, University of Greifswald, Felix-Hausdorff-Straße 4, 17489 Greifswald, Germany.; ZIK HIKE─Zentrum für Innovationskompetenz 'Humorale Immunreaktionen bei kardiovaskulären Erkrankungen', Fleischmannstraße 42, 17489 Greifswald, Germany.; DZHK (Deutsches Zentrum für Herz-Kreislauf-Forschung), Partner Site Greifswald, 17489 Greifswald, Germany. |
| Abstrakt: |
Biofilms are multicellular communities of microbial cells that grow on natural and synthetic surfaces. They have become the major cause for hospital-acquired infections because once they form, they are very difficult to eradicate. Nanotechnology offers means to fight biofilm-associated infections. Here, we report on the synthesis of silver nanoparticles (AgNPs) with the antibacterial ligand epigallocatechin gallate (EGCG) and the formation of a lysozyme protein corona on AgNPs, as shown by UV-vis, dynamic light scattering, and circular dichroism analyses. We further tested the activity of EGCG-AgNPs and their lysozyme bioconjugates on the viability of Bacillus subtilis cells and biofilm formation. Our results showed that, although EGCG-AgNPs presented no antibacterial activity on planktonic B. subtilis cells, they inhibited B. subtilis biofilm formation at concentrations larger than 40 nM, and EGCG-AgNP-lysozyme bioconjugates inhibited biofilms at concentrations above 80 nM. Cytotoxicity assays performed with human cells showed a reverse trend, where EGCG-AgNPs barely affected human cell viability while EGCG-AgNP-lysozyme bioconjugates severely hampered viability. Our results therefore demonstrate that EGCG-AgNPs may be used as noncytotoxic antibiofilm agents. |
