Identifying Mast Cells in Gastrointestinal Biopsies in Pediatric Irritable Bowel Patients.

Autor: Rizvi SA; From the Center for Digestive Health and Nutrition, Arnold Palmer Hospital for Children, Orlando, FL., Oriala C; the Department of Pathology, Orlando Health, Orlando, FL., Thomas J; From the Center for Digestive Health and Nutrition, Arnold Palmer Hospital for Children, Orlando, FL., Li S; the Department of Pathology, Orlando Health, Orlando, FL., Smadi Y; From the Center for Digestive Health and Nutrition, Arnold Palmer Hospital for Children, Orlando, FL.
Jazyk: angličtina
Zdroj: Journal of pediatric gastroenterology and nutrition [J Pediatr Gastroenterol Nutr] 2022 Nov 01; Vol. 75 (5), pp. 572-577. Date of Electronic Publication: 2022 Oct 28.
DOI: 10.1097/MPG.0000000000003588
Abstrakt: Objectives: Mast cells (MCs) have been proposed to be involved in the pathophysiology of irritable bowel syndrome (IBS). Nonetheless, the quantity and distribution of MCs in the gastrointestinal tract of pediatric patients with IBS are not well defined. This study aimed to compare the number of MCs in children with and without IBS and to establish histopathological reference values in pediatrics.
Methods: Forty-nine participants with IBS were prospectively enrolled and classified into IBS with atopy (n = 29) and IBS without atopy (n = 20). As our retrospective control group, we selected 42 individuals with a history of polyposis syndrome or gastroesophageal reflux disease with normal histopathology. Retrospective selection of the control cohort was performed in a manner similar to previously published adult and pediatric studies. MCs were prospectively stained immunohistochemically on specimens from the stomach, duodenum, terminal ileum, and descending colon of both groups.
Results: The IBS group showed significantly more MCs per high-power field (MCs/HPF) in the stomach, duodenum, terminal ileum, and descending colon ( P < 0.001), irrespective of their atopic status. Optimal MC cutoff values for IBS are ≥20.5 MCs/HPF in the stomach (area under the curve [AUC] = 0.84); ≥23.0 MCs/HPF in the duodenum (AUC = 0.79); ≥33.5 MCs/HPF in the terminal ileum (AUC = 0.82); and ≥22.5 MCs/HPF in the descending colon (AUC = 0.86).
Conclusions: Pediatric patients with IBS showed increased numbers of MCs in the stomach, duodenum, terminal ileum, and descending colon when compared with controls. Further trials are needed to explain the role of MCs in pediatric IBS, which might facilitate the development of targeted therapeutic interventions.
Competing Interests: The authors report no conflicts of interest.
(Copyright © 2022 The Author(s). Published by Wolters Kluwer on behalf of European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition.)
Databáze: MEDLINE
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