Preparation of zinc oxide nanoparticles using laser-ablation technique: Retinal epithelial cell (ARPE-19) biocompatibility and antimicrobial activity when activated with femtosecond laser.

Autor: El-Gendy AO; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt; Faculty of Pharmacy, Department of Microbiology and Immunology, Beni-Suef University, Beni-Suef 62514, Egypt., Nawaf KT; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt; Anbar Health Department, Anbar province, Ministry of Health, Iraq., Ahmed E; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt., Samir A; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt., Hamblin MR; Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein 2028, South Africa., Hassan M; Faculty of Medicine, Department of Ophthalmology, Beni-Suef University, Beni-Suef 62514, Egypt., Mohamed T; Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef 62511, Egypt. Electronic address: tarek_mohamed1969@lira.bsu.edu.eg.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2022 Sep; Vol. 234, pp. 112540. Date of Electronic Publication: 2022 Aug 12.
DOI: 10.1016/j.jphotobiol.2022.112540
Abstrakt: The unusual physical, chemical, and biological features of nanoparticles have sparked considerable attention in the ophthalmological applications. This study reports the synthesis and characterization of zinc oxide nanoparticles (ZnONPs) using laser-ablation at 100 mJ with different ablation times. The synthesized ZnONPs were spherical with an average size of 10.2 nm or 9.8 nm for laser ablation times of 20 and 30 min, respectively. The ZnONPs were screened for their antimicrobial activity against ophthalmological bacteria, methicillin-resistant S. aureus (MRSA) and Pseudomonas aeruginosa. The significant decrease in bacterial growth was observed after treatment with ZnONPs in combination with 400 nm femtosecond laser irradiation. ZnONPs were investigated for their antioxidant activity and biocompatibility towards retinal epithelial cells (ARPE-19). ZnONPs showed moderate antioxidant and free radical scavenging activity. ZnONPs prepared with an ablation time of 20 min were safer and more biocompatible than those prepared with an ablation time of 30 min, which were toxic to ARPE-19 cells with LC 50 (11.3 μg/mL) and LC 90 (18.3 μg/mL). In this study, laser ablation technique was used to create ZnONPs, and it was proposed that ZnONPs could have laser-activated antimicrobial activity for ophthalmological applications.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022. Published by Elsevier B.V.)
Databáze: MEDLINE
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