In vitro interaction of polyethylene glycol-block-poly(D,L-lactide) nanocapsule devices with host cardiomyoblasts and Trypanosoma cruzi-infective forms.

Autor: Siqueira RP; Laboratory of Pharmaceutics and Nanotechnology (LDGNano), School of Pharmacy, Federal University of Ouro Preto, Campus Universitário Morro do Cruzeiro, Ouro Preto, MG, 35400-000, Brazil. raoni.siqueira1@gmail.com.; Laboratory of Parasitic Diseases - NUPEB, Medical School, Federal University of Ouro Preto, Ouro Preto, MG, Brazil. raoni.siqueira1@gmail.com., Milagre MM; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., de Oliveira MA; Laboratory of Pharmaceutics and Nanotechnology (LDGNano), School of Pharmacy, Federal University of Ouro Preto, Campus Universitário Morro do Cruzeiro, Ouro Preto, MG, 35400-000, Brazil.; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Branquinho RT; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Torchelsen FKV; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., de Lana M; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Machado MGC; Laboratory of Pharmaceutics and Nanotechnology (LDGNano), School of Pharmacy, Federal University of Ouro Preto, Campus Universitário Morro do Cruzeiro, Ouro Preto, MG, 35400-000, Brazil.; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Andrade MS; Nanoscopy Laboratory - Technological Center (Cetec-SENAI), Belo Horizonte, MG, Brazil., Bahia MT; Laboratory of Parasitic Diseases - NUPEB, Medical School, Federal University of Ouro Preto, Ouro Preto, MG, Brazil., Mosqueira VCF; Laboratory of Pharmaceutics and Nanotechnology (LDGNano), School of Pharmacy, Federal University of Ouro Preto, Campus Universitário Morro do Cruzeiro, Ouro Preto, MG, 35400-000, Brazil. mosqueira@ufop.edu.br.; Post-graduation Program in Pharmaceutical Sciences - School of Pharmacy, Federal University of Ouro Preto, Ouro Preto, MG, Brazil. mosqueira@ufop.edu.br.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2022 Oct; Vol. 121 (10), pp. 2861-2874. Date of Electronic Publication: 2022 Aug 16.
DOI: 10.1007/s00436-022-07618-0
Abstrakt: Chagas disease, caused by the protozoan Trypanosoma cruzi, is an important public health problem in Latin America. Nanoencapsulation of anti-T. cruzi drugs has significantly improved their efficacy and reduced cardiotoxicity. Thus, we investigated the in vitro interaction of polyethylene glycol-block-poly(D,L-lactide) nanocapsules (PEG-PLA) with trypomastigotes and with intracellular amastigotes of the Y strain in cardiomyoblasts, which are the infective forms of T. cruzi, using fluorescence and confocal microscopy. Fluorescently labeled nanocapsules (NCs) were internalized by non-infected H9c2 cells toward the perinuclear region. The NCs did not induce significant cytotoxicity in the H9c2 cells, even at the highest concentrations and interacted equally with infected and non-infected cells. In infected cardiomyocytes, NCs were distributed in the cytoplasm and located near intracellular amastigote forms. PEG-PLA NCs and trypomastigote form interactions also occurred. Altogether, this study contributes to the development of engineered polymeric nanocarriers as a platform to encapsulate drugs and to improve their uptake by different intra- and extracellular forms of T. cruzi, paving the way to find new therapeutic strategies to fight the causative agent of Chagas disease.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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