Curcumin loaded hydrogel with anti-inflammatory activity to promote cartilage regeneration in immunocompetent animals.

Autor: Li X; Department of Burn and Plastic Surgery, The First Affiliated Hospital of Baotou Medical Collage, Inner Mongolia University of Science & Technology, Baotou, China., He L; Department of Rehabilitation, The First Affiliated Hospital of Baotou Medical Collage, Inner Mongolia University of Science & Technology, Baotou, China., Li N; Department of Trauma II, The First Affiliated Hospital of Baotou Medical Collage, Inner Mongolia University of Science & Technology, Baotou, China., He D; Department of Trauma Orthopedics, The First Affiliated Hospital of Baotou Medical Collage, Inner Mongolia University of Science & Technology, Baotou, China.
Jazyk: angličtina
Zdroj: Journal of biomaterials science. Polymer edition [J Biomater Sci Polym Ed] 2023 Feb; Vol. 34 (2), pp. 200-216. Date of Electronic Publication: 2022 Aug 24.
DOI: 10.1080/09205063.2022.2113290
Abstrakt: Stable cartilage regeneration in immunocompetent animals remains a huge challenge, mainly ascribing to the in vivo implantation of tissue-engineered cartilage inevitably arousing inflammatory reactions, resulting in cartilage-specific extracellular matrix erosion, chondrogenic niche destruction, and chondrocyte deterioration. Herein, we developed an anti-inflammatory platform, namely, Cur/GelMA hydrogel, by loading a potent anti-inflammatory drug of curcumin (Cur) into gelatin methacryloyl (GelMA) hydrogel. The Cur/GelMA hydrogel exhibited satisfactory Cur release kinetics in vitro and exerted favorable anti-inflammatory effects when cocultured with lipopolysaccharide-induced RAW264.7 macrophages in vitro . Furthermore, the Cur/GelMA hydrogel showed gratifying biocompatibility and supported cartilage regeneration in vitro when colonized with rabbit- and goat-derived chondrocytes. In addition, the in vitro engineered cartilages in the Cur/GelMA hydrogel were able to maintain a cartilaginous phenotype and achieved stable cartilage regeneration when subcutaneously implanted in autologous rabbits and goats for 2 and 4 weeks compared to the GelMA hydrogel. Furthermore, our data revealed that the in vivo -generated cartilage in the Cur/GelMA group apparently alleviated the inflammatory reaction compared to its GelMA counterpart, suggesting that the locally released Cur endowed the Cur/GelMA hydrogel with potent anti-inflammatory capacity. This study provides a reliable anti-inflammatory platform for stable cartilage regeneration in immunocompetent animals, significantly advancing the clinical application of tissue-engineered cartilage.
Databáze: MEDLINE
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