| Autor: |
Berden JH, Hagemann JF, Koene RA |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of immunological methods [J Immunol Methods] 1978; Vol. 23 (1-2), pp. 149-59. |
| DOI: |
10.1016/0022-1759(78)90118-7 |
| Abstrakt: |
Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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