Basophil Depletion Alters Host Immunity, Intestinal Permeability, and Mammalian Host-to-Mosquito Transmission in Malaria.

Autor:	Donnelly EL; Department of Biological Sciences, University of Idaho, Moscow, ID., Céspedes N; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and., Hansten G; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and., Wagers D; Department of Biological Sciences, University of Idaho, Moscow, ID., Briggs AM; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and., Lowder C; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and., Schauer J; Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, Davis, CA., Garrison SM; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and., Haapanen L; Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, Davis, CA., Van de Water J; Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, Davis, CA., Luckhart S; Department of Biological Sciences, University of Idaho, Moscow, ID; sluckhart@uidaho.edu.; Department of Entomology, Plant Pathology and Nematology, University of Idaho, Moscow, ID; and.
Jazyk:	angličtina
Zdroj:	ImmunoHorizons [Immunohorizons] 2022 Aug 15; Vol. 6 (8), pp. 581-599. Date of Electronic Publication: 2022 Aug 15.
DOI:	10.4049/immunohorizons.2200055
Abstrakt:	Malaria-induced bacteremia has been shown to result from intestinal mast cell (MC) activation. The appearance of MCs in the ileum and increased intestinal permeability to enteric bacteria are preceded by an early Th2-biased host immune response to infection, characterized by the appearance of IL-4, IL-10, mast cell protease (Mcpt)1 and Mcpt4, and increased circulating basophils and eosinophils. Given the functional similarities of basophils and MCs in the context of allergic inflammation and the capacity of basophils to produce large amounts of IL-4, we sought to define the role of basophils in increased intestinal permeability, in MC influx, and in the development of bacteremia in the context of malaria. Upon infection with nonlethal Plasmodium yoelii yoelii 17XNL, Basoph8 × ROSA-DTα mice or baso (-) mice that lack basophils exhibited increased intestinal permeability and increased ileal MC numbers, without any increase in bacterial 16S ribosomal DNA copy numbers in the blood, relative to baso (+) mice. Analysis of cytokines, chemokines, and MC-associated factors in the ileum revealed significantly increased TNF-α and IL-13 at day 6 postinfection in baso (-) mice compared with baso (+) mice. Moreover, network analysis of significantly correlated host immune factors revealed profound differences between baso (-) and baso (+) mice following infection in both systemic and ileal responses to parasites and translocated bacteria. Finally, basophil depletion was associated with significantly increased gametocytemia and parasite transmission to Anopheles mosquitoes, suggesting that basophils play a previously undescribed role in controlling gametocytemia and, in turn, mammalian host-to-mosquito parasite transmission. (Copyright © 2022 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu