Transcriptome responses in copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus exposed to phenanthrene and benzo[a]pyrene.

Autor: Yadetie F; Department of Biological Sciences, University of Bergen, Bergen, Norway. Electronic address: Fekadu.Yadetie@uib.no., Brun NR; Department of Biological Sciences, University of Bergen, Bergen, Norway; Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA, USA. Electronic address: Nadja.Brun@uib.no., Giebichenstein J; Department of Biosciences, University of Oslo, Oslo, Norway. Electronic address: julia.giebichenstein@ibv.uio.no., Dmoch K; Oithona, Katarzyna Dmoch Co, Gdańsk, Poland., Hylland K; Department of Biosciences, University of Oslo, Oslo, Norway. Electronic address: ketil.hylland@ibv.uio.no., Borgå K; Department of Biosciences, University of Oslo, Oslo, Norway. Electronic address: katrine.borga@ibv.uio.no., Karlsen OA; Department of Biological Sciences, University of Bergen, Bergen, Norway. Electronic address: Odd.Karlsen@uib.no., Goksøyr A; Department of Biological Sciences, University of Bergen, Bergen, Norway. Electronic address: Anders.Goksoyr@uib.no.
Jazyk: angličtina
Zdroj: Marine genomics [Mar Genomics] 2022 Oct; Vol. 65, pp. 100981. Date of Electronic Publication: 2022 Aug 12.
DOI: 10.1016/j.margen.2022.100981
Abstrakt: Arctic and sub-arctic pelagic organisms can be exposed to effluents and spills from offshore petroleum-related activities and thus it is important to understand how they respond to crude oil related contaminants such as polycyclic aromatic hydrocarbons (PAHs). The copepod species Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus represent key links in the arctic marine food web. We performed a transcriptome analysis of the three species exposed to phenanthrene (Phe) and benzo[a]pyrene (BaP) representing low and high molecular weight PAHs, respectively. Differential expression of several genes involved in many cellular pathways was observed after 72 h exposure to Phe (0.1 μM) and BaP (0.1 μM). In C. finmarchicus and C. glacialis, the exposure resulted in up-regulation of genes encoding enzymes in xenobiotic biotransformation, particularly the phase II cytosolic sulfonation system that include 3'-phosphoadenosine 5'-phosphosulfate synthase (PAPSS) and sulfotransferases (SULTs). The sulfonation pathway genes were more strongly induced by BaP than Phe in C. finmarchicus and C. glacialis but were not affected in C. hyperboreus. However, a larger number of genes and pathways were modulated in C. hyperboreus by the PAHs including genes encoding xenobiotic biotransformation and lipid metabolism enzymes, suggesting stronger responses in this species. The results suggest that the cytosolic sulfonation is a major phase II conjugation pathway for PAHs in C. finmarchicus and C. glacialis. Some of the biotransformation systems affected are known to be involved in metabolism of endogenous compounds such as ecdysteroids, which may suggest potential interference with physiological and developmental processes of the copepod species.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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