Comparison of Sample Preparation Techniques for the (-)ESI-FT-ICR-MS Analysis of Humic and Fulvic Acids.

Autor:	Goranov AI; Department of Chemistry and Biochemistry, Old Dominion University, 4501 Elkhorn Avenue, Norfolk, Virginia 23529, United States., Tadini AM; Department of Chemistry and Biochemistry, Old Dominion University, 4501 Elkhorn Avenue, Norfolk, Virginia 23529, United States.; Embrapa Instrumentação, Brazilian Agricultural Research Corporation - Embrapa, Street XV de Novembro, 1452, São Carlos, São Paulo 13560-970, Brazil., Martin-Neto L; Embrapa Instrumentação, Brazilian Agricultural Research Corporation - Embrapa, Street XV de Novembro, 1452, São Carlos, São Paulo 13560-970, Brazil., Bernardi ACC; Embrapa Pecuária Sudeste, Brazilian Agricultural Research Corporation - Embrapa, Rodovia Washington Luiz, Km 234 s/n°, Fazenda Canchim São Carlos, São Paulo 13560-970, Brazil., Oliveira PPA; Embrapa Pecuária Sudeste, Brazilian Agricultural Research Corporation - Embrapa, Rodovia Washington Luiz, Km 234 s/n°, Fazenda Canchim São Carlos, São Paulo 13560-970, Brazil., Pezzopane JRM; Embrapa Pecuária Sudeste, Brazilian Agricultural Research Corporation - Embrapa, Rodovia Washington Luiz, Km 234 s/n°, Fazenda Canchim São Carlos, São Paulo 13560-970, Brazil., Milori DMBP; Embrapa Instrumentação, Brazilian Agricultural Research Corporation - Embrapa, Street XV de Novembro, 1452, São Carlos, São Paulo 13560-970, Brazil., Mounier S; Unité mixte 110, Mediterranean Institute of Oceanography (MIO), Université de Toulon, Avenue de l'Université - Bat. R, Toulon 83041, France.; Unité mixte 110, MIO, Aix Marseille Université, 163 Avenue de Luminy, Marseille 13288, France.; Unité mixte 110, MIO, Institut de la Recherche et du Développement, 163 Avenue de Luminy, Marseille 13288, France.; Unité mixte 110, MIO, Institut des Sciences de l'Univers, Centre National de la Recherche Scientifique, 163 Avenue de Luminy, Marseille 13288, France., Hatcher PG; Department of Chemistry and Biochemistry, Old Dominion University, 4501 Elkhorn Avenue, Norfolk, Virginia 23529, United States.
Jazyk:	angličtina
Zdroj:	Environmental science & technology [Environ Sci Technol] 2022 Sep 06; Vol. 56 (17), pp. 12688-12701. Date of Electronic Publication: 2022 Aug 15.
DOI:	10.1021/acs.est.2c01125
Abstrakt:	Soil organic matter (SOM) plays a key role in the global carbon and nitrogen cycles. Soil biogeochemistry is regularly studied by extracting the base-soluble fractions of SOM: acid-insoluble humic acid (HA) and acid-soluble fulvic acid (FA). Electrospray ionization-Fourier transform-ion cyclotron resonance-mass spectrometry (ESI-FT-ICR-MS) is commonly utilized for molecularly characterizing these fractions. Different sample preparation techniques exist for the analysis of HA and FA though questions remain regarding data comparability following different preparations. Comparisons of different sample preparation techniques here revealed that the negative-mode ESI-FT-ICR-MS analytical window can be skewed to detect different groups of molecules, with primary differences in oxygenation, aromaticity, and molecular weight. It was also observed that HA and FA from soils versus an aquatic matrix behaved very differently. Thus, we conclude that sample preparation techniques determined to be "most optimal" in our study are in no way universal. We recommend that future studies of HA and FA involve similar comparative studies for determining the most suitable sample preparation technique for their particular type of HA or FA matrices. This will enhance data comparability among different studies and environmental systems and ultimately allow us to better understand the complex composition of environmental matrices.
Databáze:	MEDLINE
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