Deciphering the Emulsification Process to Create an Albumin-Perfluorocarbon-(o/w) Nanoemulsion with High Shelf Life and Bioresistivity.

Autor: Jaegers J; University of Duisburg-Essen, Institute of Physiology, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany.; Department of Biomedicine, Aarhus University, Høegh-Guldbergs Gade 10, bygning 1116, 8000 Aarhus C, Denmark., Haferkamp S; SOLID-CHEM GmbH, Universitätsstraße 136, 44799 Bochum, Germany., Arnolds O; Biomolecular Spectroscopy and RUBiospek|NMR, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstraße 150, 44780 Bochum, Germany., Moog D; Pulveranalyse Dipl.-Ing. Daniel Moog, Roitzheimer Str. 61, 53879 Euskirchen, Germany., Wrobeln A; University of Duisburg-Essen, Institute of Physiology, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany., Nocke F; University of Duisburg-Essen, Institute of Physiology, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany., Cantore M; University of Duisburg-Essen, Institute of Physiology, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany., Pütz S; Biomolecular Spectroscopy and RUBiospek|NMR, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstraße 150, 44780 Bochum, Germany., Hartwig A; Physical Chemistry-innoFSPEC and Potsdam Transfer, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany., Franzkoch R; CellNanOs (Center of Cellular Nanoanalytics), iBiOs (Integrated Bioimaging Facility), University of Osnabrück, Barbarastr. 11, 49076 Osnabrück, Germany., Psathaki OE; CellNanOs (Center of Cellular Nanoanalytics), iBiOs (Integrated Bioimaging Facility), University of Osnabrück, Barbarastr. 11, 49076 Osnabrück, Germany., Jastrow H; Institute of Anatomy, University of Duisburg-Essen, University Hospital Essen, Hufelandstr. 55, Essen D-45147, Germany.; Institute for Experimental Immunology and Imaging, Imaging Center Essen, Electron Microscopy Unit, University of Duisburg-Essen, Hufelandstr. 55, Essen D-45147, Germany., Schauerte C; SOLID-CHEM GmbH, Universitätsstraße 136, 44799 Bochum, Germany., Stoll R; Biomolecular Spectroscopy and RUBiospek|NMR, Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Universitätsstraße 150, 44780 Bochum, Germany., Kirsch M; University of Duisburg-Essen, Institute of Physiological Chemistry, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany., Ferenz KB; University of Duisburg-Essen, Institute of Physiology, University Hospital Essen, Hufelandstraße 55, 45122 Essen, Germany.; CeNIDE (Center for Nanointegration Duisburg-Essen) University of Duisburg-Essen, Carl-Benz-Strasse 199, 47057 Duisburg, Germany.
Jazyk: angličtina
Zdroj: Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2022 Aug 30; Vol. 38 (34), pp. 10351-10361. Date of Electronic Publication: 2022 Aug 15.
DOI: 10.1021/acs.langmuir.1c03388
Abstrakt: This work aimed at the development of a stable albumin-perfluorocarbon (o/w) emulsion as an artificial oxygen carrier suitable for clinical application. So far, albumin-perfluorocarbon-(o/w) emulsions have been successfully applied in preclinical trials. Cross-linking a variety of different physical and chemical methods for the characterization of an albumin-perfluorocarbon (PFC)-(o/w) emulsion was necessary to gain a deep understanding of its specific emulsification processes during high-pressure homogenization. High-pressure homogenization is simple but incorporates complex physical reactions, with many factors influencing the formation of PFC droplets and their coating. This work describes and interprets the impact of albumin concentration, homogenization pressure, and repeated microfluidizer passages on PFC-droplet formation; its influence on storage stability; and the overcoming of obstacles in preparing stable nanoemulsions. The applied methods comprise dynamic light scattering, static light scattering, cryo- and non-cryo-scanning and transmission electron microscopies, nuclear magnetic resonance spectroscopy, light microscopy, amperometric oxygen measurements, and biochemical methods. The use of this wide range of methods provided a sufficiently comprehensive picture of this polydisperse emulsion. Optimization of PFC-droplet formation by means of temperature and pressure gradients results in an emulsion with improved storage stability (tested up to 5 months) that possibly qualifies for clinical applications. Adaptations in the manufacturing process strikingly changed the physical properties of the emulsion but did not affect its oxygen capacity.
Databáze: MEDLINE