Immunoinformatics design of multi-epitope peptide for the diagnosis of Schistosoma haematobium infection.

Autor: Oladipo EK; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Laboratory of Molecular Biology, Bioinformatics and Immunology, Department of Microbiology, Adeleke University, Ede, Osun State, Nigeria., Jimah EM; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria., Irewolede BA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria., Folakanmi EO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Olubodun OA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Adediran DA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Akintibubo SA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Odunlami FD; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Olufemi SE; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Ojo TO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Akinro OP; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Hezekiah OS; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Olayinka AT; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Abiala GA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Idowu AF; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Ogunniran JA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Medical Microbiology and Parasitology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Ikuomola MO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Adegoke HM; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Laboratory of Computational and Biophysical Chemistry, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Idowu UA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Akindiya OE; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Biology, Olusegun Agagu University of Science and Technology, Okiti-pupa, Ondo State, Nigeria., Oluwasanya GJ; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Laboratory of Molecular Biology, Bioinformatics and Immunology, Department of Microbiology, Adeleke University, Ede, Osun State, Nigeria., Akanbi GM; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Bamigboye FO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria., Aremu RO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Laboratory of Molecular Biology, Bioinformatics and Immunology, Department of Microbiology, Adeleke University, Ede, Osun State, Nigeria., Awobiyi HO; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Laboratory of Molecular Biology, Bioinformatics and Immunology, Department of Microbiology, Adeleke University, Ede, Osun State, Nigeria., Kolapo KT; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Oluwasegun JA; Genomics Unit, Helix Biogen Institute, Ogbomoso, Oyo State, Nigeria.; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria., Olatunde SK; Department of Microbiology and Pathology, All Saints University School of Medicine, Roseau, Commonwealth of Dominica, West Indies., Adelusi TI; Computational Biology/Drug discovery laboratory, Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Jazyk: angličtina
Zdroj: Journal of biomolecular structure & dynamics [J Biomol Struct Dyn] 2023 Aug-Sep; Vol. 41 (14), pp. 6676-6683. Date of Electronic Publication: 2022 Aug 13.
DOI: 10.1080/07391102.2022.2111358
Abstrakt: Schistosoma haematobium has been identified as a significant cause of urogenital disease, as well as a risk factor for bladder cancer and HIV/AIDS. The parasites are obtained trans-dermally by swimming or wading in contaminated freshwater, and they are also transmitted to humans by freshwater snails. The organisms infect the vasculature of the gastrointestinal or genitourinary tracts. Worms live in blood vessels and lay eggs that become embedded in the bladder wall, causing chronic immune-mediated disease and squamous cell carcinoma growth. The primary goal of this research is to predict and design a novel synthetic protein containing multiple immunodominant B cell epitopes using three schistosome proteins: XP-012801068.2, XP-012801892.2, and XP-012793835.2 softwares were used to analyze the proteins' primary, secondary, and tertiary structures (BepiPred, BcPred).The B cell construct was then evaluated using I-TASSER server, and physicochemical properties, as well as homology modeling of the 3 D structure of the protein, was obtained. In silico analyses revealed regions with high immunogenicity. For XP-012801068.2, three epitopes are found between residues 292-334, 3-22, and 314-333; for XP-012801892.2, three epitopes are found in the residues 184-236, 81-100, and 329-348 for XP-012793835.2, four epitopes are found in the residues 185-222, 469-512, 649-713, and 338-357. The construct's has an average length of 308 bp, instability index of 49.96, theoretical PI of 4.2 and a C score -1.59. Furthermore, these parameters analyzed reveals that the constructed multi-epitope peptide has the potential to provide a theoretical basis for the development of a Schistosoma haematobium diagnostic kit.Communicated by Ramaswamy H. Sarma.
Databáze: MEDLINE