Evaluation of methicillin-resistant Staphylococcus virulence genes and antibiotics susceptibility in Iranian population.

Autor: Armin S; Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Karimi A; Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Pourmoghaddas Z; Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran.; Department of Pediatrics, Isfahan University of Medical Sciences, Isfahan, Iran., Azimi L; Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Fallah F; Pediatric Infections Research Center, Research Institute for Children Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Tahbaz SV; Clinical Cancer Research Center, Milad General Hospital, Tehran, Iran.
Jazyk: angličtina
Zdroj: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences [J Res Med Sci] 2022 May 30; Vol. 27, pp. 36. Date of Electronic Publication: 2022 May 30 (Print Publication: 2022).
DOI: 10.4103/jrms.JRMS_543_19
Abstrakt: Background: Methicillin resistance Staphylococcus aureus (MRSA) is one most important pathogens for human health. The ability of this organism for producing different kinds of disease is related to its virulence gene. The frequency of hemolysin alpha (hla), hemolysin beta (hlb), and exfoliative toxin A (eta) virulence genes of MRSA was evaluated, and the association of these genes with antibiotics susceptibility was investigated.
Materials and Methods: In a cross-sectional study, a total of 695 Staphylococcus clinical samples from seven different provinces of Iran were evaluated. MRSA was detected by cefoxitin disk. Virulence genes were detected by polymerase chain reaction. Susceptibility to clindamycin and ciprofloxacin was evaluated according to the Clinical and Laboratory Standards Institute guideline.
Results: From a total of 695 samples, 170 (24.46%) were found to be MRSA. 142, 82, and 132 samples of MRSA were hla, hlb, and eta positive, respectively. hla gene was significantly found more frequently in patients at least 18 years ( P = 0.02). 105 (68.6%) and 93 (59.6%) of MRSA samples were resistance to ciprofloxacin and clindamycin, respectively. hlb gene was significantly more resistant to clindamycin ( P = 0.04) and ciprofloxacin ( P = 0.01). Logistic regression analysis displayed hlb-positive MRSA strains were significantly associated with ciprofloxacin (odds ratio [OR]: 3.6, 95% confidence interval [CI] = 1.637-8.00) and clindamycin (OR: 1.93, 95% CI 1.00-3.68).
Conclusion: MRSA strains from Staphylococcus aureus which isolated from hospitalized Iranian patients are significantly resistant to clindamycin and ciprofloxacin and it is may be because of hlb virulence gene. These samples consist of both community-acquired MRS) and health-care associated MRSA, so we could not use this finding as a guide for local antibiotics usage.
Competing Interests: There are no conflicts of interest.
(Copyright: © 2022 Journal of Research in Medical Sciences.)
Databáze: MEDLINE
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