Endophytic and rhizospheric bacteria associated with Paspalum atratum and its potential for plant growth promotion with different phosphate sources.

Autor:	de Paula AF; Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil., Cruz FPN; Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Biotechnology Graduate Program, Exact and Technology Sciences Center, Federal University of São Carlos, São Carlos, Brazil., Dinato NB; Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Embrapa Pecuária Sudeste, São Carlos, Brazil., de Andrade PHM; Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil., de Moraes ACP; Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Biotechnology Graduate Program, Exact and Technology Sciences Center, Federal University of São Carlos, São Carlos, Brazil., Junior WB; Embrapa Pecuária Sudeste, São Carlos, Brazil., Bernardi ACC; Embrapa Pecuária Sudeste, São Carlos, Brazil., Vigna BBZ; Embrapa Pecuária Sudeste, São Carlos, Brazil., Fávero AP; Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Embrapa Pecuária Sudeste, São Carlos, Brazil., Lacava PT; Laboratory of Microbiology and Biomolecules, Department of Morphology and Pathology, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Evolutionary Genetics and Molecular Biology Graduate Program, Biological and Health Sciences Center, Federal University of São Carlos, São Carlos, Brazil.; Biotechnology Graduate Program, Exact and Technology Sciences Center, Federal University of São Carlos, São Carlos, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Jul 28; Vol. 13, pp. 884716. Date of Electronic Publication: 2022 Jul 28 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.884716
Abstrakt:	The genus Paspalum belongs to the family Poaceae and has several species that are native to Brazil. The Paspalum Germplasm Bank (GB) of the Brazilian Agricultural Research Corporation comprises approximately 450 accessions from 50 species. Among these accessions, Paspalum atratum (BGP 308) has economic potential for forage purposes. However, the endophytic and rhizospheric microbial communities within this accession and their ability to promote plant growth remain unknown. The present study aimed to isolate the endophytic and rhizospheric bacteria associated with P. atratum and to assess their potential for plant growth improvement, so-called plant growth-promoting bacteria (PGPB). For the in vitro tests, the ability of nitrogen-fixing bacteria (NFB), phosphate solubilization (PS) and indoleacetic acid (IAA) production were evaluated. A total of 116 endophytic and rhizosphere bacteria were obtained from the isolation. In the in vitro tests, 43 (37.00%) of these isolates showed positive NFB, PS, and IAA results. These isolates were identified by 16S rDNA sequencing. The phosphate solubilization index (PSI) ranged from 2 to 3.61, all 43 strains performed biological nitrogen fixation and the IAA production ranged from 12.85 to 431.41 μg ml -1 . Eight of these 43 isolates were evaluated in vivo in a greenhouse using P. atratum caryopsis. The pots were filled with soil prepared with three different phosphate sources and one control without phosphate. After growth, the plants were submitted to morphological, bromatological and chemical determination. Data were analyzed using analysis of variance (ANOVA) and principal component analysis (PCA). In the in vivo test, treatments 105 ( Pseudomonas sp.) and 458 ( Pseudomonas sp.) were the most significant for the crystalline phosphate source, 109 ( Bacillus sp.) for the sedimentary phosphate source and, as for the soluble phosphate source most treatments that received bacterial isolates had higher phosphorus content in the dry matter than the uninoculated soluble phosphate control. The 105FCR (crystalline phosphate +  Pseudomonas sp.), 109FSE (sedimentary phosphate +  Bacillus sp.), and 110 FSE (sedimentary phosphate +  Enterobacter sp.) treatments showed the best results for plant growth promotion. This work made it possible to determine the bacterial community associated with P. atratum (BGP308) and to obtain new potential plant growth-promoting strains. Competing Interests: ND, WJ, AB, BV, and AF were employed by Embrapa Pecuária Sudeste. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 de Paula, Cruz, Dinato, de Andrade, de Moraes, Junior, Bernardi, Vigna, Fávero and Lacava.)
Databáze:	MEDLINE
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