Production and Optimization of Conjugated Linoleic and Eicosapentaenoic Acids by Bifidobacterium lactis in Cold-Pressed Soybean Cake.

Autor:	Azari SR; Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Hojjatoleslamy M; Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran., Mousavi ZE; Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.; Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran., Kiani H; Department of Food Science and Technology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.; Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, Campus of Agriculture and Natural Resources, University of Tehran, Karaj, Iran., Jalali SMA; Department of Animal Sciences, Faculty of Agriculture and Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.; Research Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Jazyk:	angličtina
Zdroj:	Frontiers in nutrition [Front Nutr] 2022 Jul 27; Vol. 9, pp. 916728. Date of Electronic Publication: 2022 Jul 27 (Print Publication: 2022).
DOI:	10.3389/fnut.2022.916728
Abstrakt:	Background and Purpose: In regard to the biosynthesis of conjugated linoleic acid (CLA) and eicosapentaenoic acid (EPA) by some bacteria, the objective of this study was to evaluate the efficiency of solid-state fermentation based on soybean pressed cake (SPC) to produce CLA and EPA by Bifidobacterium lactis . The objective of this study was to evaluate the efficiency of solid-state fermentation based on SPC to produce CLA and EPA by B. lactis . Methods: Process conditions including humidity, inoculation level, and temperature parameters were optimized by adopting the response surface methodology (RSM) method (response surface method) and the design expert software. Accordingly, a homogeneous SPC paste substrate at 60, 70, and 80% humidity was prepared with different inoculation levels at 30, 37, and 44°C to assess the strain behavior. The introduced SPC consisted of 60% humidity, 2% inoculation level at 37°C, and 60% humidity, and 4% inoculation level at 30 and 44°C; it also included 6% inoculation level at 37°C, 70% humidity at 2% inoculation level, at 30 and 44°C, and 4% inoculation level at 37°C. Also, SPC with 80% humidity at 2% and 4% inoculation levels, and at 30 and 44°C was obtained. To confirm the accuracy of the conditions, an experiment was conducted according to the defined requirements. Results: The results were compared with the predicted data, which showed a significant difference. Under optimized conditions, with an inoculation level of 4% on the SPC medium with 70% humidity and at 37°C, B. lactis strains could yield 9 cis- , 11 trans- linoleic and eicosapentaenoic at 0.18 and 0.39% of the total fatty acids. Conclusion: So, the potential benefits of using SPC as an inexpensive substrate for the commercial production of CLA and EPA should be noted. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Azari, Hojjatoleslamy, Mousavi, Kiani and Jalali.)
Databáze:	MEDLINE
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