Consumption of soy isoflavones during the prepubertal phase delays puberty and causes hypergonadotropic hypogonadism with disruption of hypothalamic-pituitary gonadotropins regulation in male rats.

Autor: Oliveira JM; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: jeaneoli.m@gmail.com., Oliveira IM; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: medeiros_isabela@hotmail.com., Sleiman HK; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: hanansleiman1987@gmail.com., Dal Forno GO; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: gonzaloodf@gmail.com., Romano MA; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: maromano17@unicentro.br., Romano RM; Laboratory of Reproductive Toxicology, Department of Medicine, State University of Centro-Oeste (UNICENTRO), Rua Simeão Camargo Varela de Sa, 03, Zip-Code 85040-080, Parana, Brazil. Electronic address: romano@unicentro.br.
Jazyk: angličtina
Zdroj: Toxicology letters [Toxicol Lett] 2022 Oct 01; Vol. 369, pp. 1-11. Date of Electronic Publication: 2022 Aug 11.
DOI: 10.1016/j.toxlet.2022.08.004
Abstrakt: Isoflavones are phytoestrogens with recognized estrogenic activity but may also affect testosterone, corticosterone and thyroid hormone levels in experimental models. However, the molecular mechanisms involved in these alterations are still unclear. Isoflavones are present in soy-based infant formula, in breast milk after the consumption of soy by the mother and are widely used for the preparation of beverages consumed by toddlers and teenagers. In this sense, we proposed to investigate the effects of soy isoflavone exposure during the prepubertal period, a recognized window of sensitivity for endocrine disruption, over the hypothalamic-pituitary-testicular (HPT) axis. For this, 42 3-week-old male Wistar rats were exposed to 0.5, 5 or 50 mg of soy isoflavones/kg from postnatal day (PND) 23 to PND60. We evaluated body growth, age at puberty, serum concentrations of LH, FSH, testosterone and estradiol, and the expression of the transcripts (mRNA) of genes encoding key genes controlling the hypothalamic-pituitary-testicular (HPT) axis. In the hypothalamus, we observed an increase in Esr1 mRNA expression (0.5 and 5 mg). In the pituitary, we observed an increase in Gnrhr mRNA expression (50 mg), a reduction in Lhb mRNA expression (0.5 mg), and a reduction in Ar mRNA expression. In the testis, we observed an increase in Lhcgr mRNA expression (50 mg) and a reduction in Star mRNA expression (0.5 and 5 mg). The serum levels of LH (5 and 50 mg) and FSH (0.5 mg) were increased, while testosterone and estradiol were reduced. Puberty was delayed in all groups. Taken together, these results suggest that prepubertal consumption of relevant levels of soy isoflavones disrupts the HPT axis, causing hypergonadotropic hypogonadism and altered expression levels of key genes regulating the axis.
Competing Interests: Declaration of Competing Interest The authors declare that there are no conflicts of interest to disclose.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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