Reproductive toxicity of maternal exposure to di(2-ethylhexyl)phthalate and butyl paraben (alone or in association) on both male and female Wistar offspring.

Autor: Guerra MT; Federal University of Mato Grosso do Sul, Campus Três Lagoas, Três Lagoas, Brazil., Erthal RP; Department of Pathological Sciences, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Punhagui-Umbelino APF; Department of Pathological Sciences, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Trinque CM; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Torres de Bari MA; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Nunes TDM; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Costa WF; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Cleto PH; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil., Fernandes GSA; Department of General Biology, Biological Sciences Center, State University of Londrina-UEL, Londrina, Brazil.
Jazyk: angličtina
Zdroj: Journal of applied toxicology : JAT [J Appl Toxicol] 2023 Feb; Vol. 43 (2), pp. 242-261. Date of Electronic Publication: 2022 Aug 24.
DOI: 10.1002/jat.4377
Abstrakt: Parabens and phthalates are commonly found as contaminants in human fluids and are able to provoke reproductive toxicity, being considered endocrine disruptors. To evaluate the effects of phthalate and paraben, alone or in combination, on reproductive development of the offspring, female pregnant Wistar rats were allocated in six experimental groups: Three control groups (gavage [CG], subcutaneous [CS], and gavage + subcutaneous) received corn oil as vehicle, and the remaining groups were exposed to di(2-ethylhexyl)phthalate (DEHP) (500 mg/kg, gavage), butyl paraben (BP) (100 mg/kg, subcutaneously), or MIX (DEHP + BP), from Gestational Day 12 until Postnatal Day (PND) 21. The following parameters were assessed on the offspring: anogenital distance and weight at PND 1, nipple counting at PND 13, puberty onset, estrous cycle, weights of reproductive and detoxifying organs, histological evaluation of reproductive organs, and sperm evaluations (counts, morphology, and motility). Female pups from MIX group presented reduced body weight at PND 1, lower AGD, and decreased endometrium thickness. Male animals showed decreased body weight at PND 1 and lower number of Sertoli cells on DEHP and MIX groups, MIX group revealed increase of abnormal seminiferous tubules, DEHP animals presented delayed preputial separation and higher percentage of immotile sperms, and BP males presented diminished number of Leydig cells. In conclusion, the male offspring was more susceptible to DEHP toxicity; even when mixed to paraben, the main negative effects observed seem to be due to antiandrogenic phthalate action. On the other hand, DEHP seems to be necessary to improve the effects of BP on reducing estrogen-dependent and increasing androgen-dependent events.
(© 2022 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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