Protein diffusion in Escherichia coli cytoplasm scales with the mass of the complexes and is location dependent.

Autor:	Śmigiel WM; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands., Mantovanelli L; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands., Linnik DS; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands., Punter M; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands., Silberberg J; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands., Xiang L; Department of Chemistry, UC Berkeley, Stanley Hall, Berkeley, CA 94720, USA., Xu K; Department of Chemistry, UC Berkeley, Stanley Hall, Berkeley, CA 94720, USA., Poolman B; Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands.
Jazyk:	angličtina
Zdroj:	Science advances [Sci Adv] 2022 Aug 12; Vol. 8 (32), pp. eabo5387. Date of Electronic Publication: 2022 Aug 12.
DOI:	10.1126/sciadv.abo5387
Abstrakt:	We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing Escherichia coli . We evaluate the method for application in small compartments and find that confining effects of the cell membrane affect the diffusion maps. Our analysis reveals that protein diffusion at the poles is consistently slower than in the center of the cell, i.e., to an extent greater than the confining effect of the cell membrane. We also show that the diffusion coefficient scales with the mass of the used probes, taking into account the oligomeric state of the proteins, while parameters such as native protein abundance or the number of protein-protein interactions do not correlate with the mobility of the proteins. We argue that our data paint the prokaryotic cytoplasm as a compartment with subdomains in which the diffusion of macromolecules changes with the perceived viscosity.
Databáze:	MEDLINE
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