Dedicated farnesyl diphosphate synthases circumvent isoprenoid-derived growth-defense tradeoffs in Zea mays.

Autor: Tang HV; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Berryman DL; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA.; Department of Horticultural Sciences, University of Florida, Gainesville, FL, USA., Mendoza J; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Yactayo-Chang JP; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Li QB; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Christensen SA; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Hunter CT; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA., Best N; Plant Genetics Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Columbia, MO, USA., Soubeyrand E; Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, Canada., Akhtar TA; Molecular and Cellular Biology Department, University of Guelph, Guelph, ON, Canada., Basset GJ; Department of Horticultural Sciences, University of Florida, Gainesville, FL, USA., Block AK; Chemistry Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology, Gainesville, FL, USA.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2022 Oct; Vol. 112 (1), pp. 207-220. Date of Electronic Publication: 2022 Aug 31.
DOI: 10.1111/tpj.15941
Abstrakt: Zea mays (maize) makes phytoalexins such as sesquiterpenoid zealexins, to combat invading pathogens. Zealexins are produced from farnesyl diphosphate in microgram per gram fresh weight quantities. As farnesyl diphosphate is also a precursor for many compounds essential for plant growth, the question arises as to how Z. mays produces high levels of zealexins without negatively affecting vital plant systems. To examine if specific pools of farnesyl diphosphate are made for zealexin synthesis we made CRISPR/Cas9 knockouts of each of the three farnesyl diphosphate synthases (FPS) in Z. mays and examined the resultant impacts on different farnesyl diphosphate-derived metabolites. We found that FPS3 (GRMZM2G098569) produced most of the farnesyl diphosphate for zealexins, while FPS1 (GRMZM2G168681) made most of the farnesyl diphosphate for the vital respiratory co-factor ubiquinone. Indeed, fps1 mutants had strong developmental phenotypes such as reduced stature and development of chlorosis. The replication and evolution of the fps gene family in Z. mays enabled it to produce dedicated FPSs for developmentally related ubiquinone production (FPS1) or defense-related zealexin production (FPS3). This partitioning of farnesyl diphosphate production between growth and defense could contribute to the ability of Z. mays to produce high levels of phytoalexins without negatively impacting its growth.
(© 2022 Society for Experimental Biology and John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA.)
Databáze: MEDLINE
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