| Autor: |
Hayter EA; Department of Chemistry, Saint Louis University, 3501 Laclede Ave St. Louis, MO, USA, 63103. scott.martin@slu.edu., Azibere S; Department of Chemistry, Saint Louis University, 3501 Laclede Ave St. Louis, MO, USA, 63103. scott.martin@slu.edu., Skrajewski LA; Department of Biomedical Engineering, Institute for Quantitative Health Science & Engineering, Michigan State University, USA., Soule LD; Department of Biomedical Engineering, Institute for Quantitative Health Science & Engineering, Michigan State University, USA., Spence DM; Department of Biomedical Engineering, Institute for Quantitative Health Science & Engineering, Michigan State University, USA., Martin RS; Department of Chemistry, Saint Louis University, 3501 Laclede Ave St. Louis, MO, USA, 63103. scott.martin@slu.edu.; Center for Additive Manufacturing, Saint Louis University, USA. |
| Abstrakt: |
In this paper, a 3D-printed multi-modal device was designed and fabricated to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions prepared from whole blood. Once a sample was injected into the device, NO was first detected ( via amperometry) using a three-electrode, dual-opposed, electrode configuration with a platinum-black/Nafion coated gold working electrode. After in-line amperometric detection of NO, ATP was detected via a chemiluminescence reaction, with a luciferin/luciferase solution continuously pumped into an integrated mixing T and the resulting light being measured with a PMT underneath the channel. The device was optimized for mixing/reaction conditions, limits of detection (40 nM for NO and 30 nM for ATP), and sensitivity. This device was used to determine the basal (normoxic) levels of NO and ATP in red blood cells, as well as an increase in concentration of both analytes under hypoxic conditions. Finally, the effect of storing red blood cells in a commonly used storage solution was also investigated by monitoring the production of NO and ATP over a three-week storage time. |
