| Autor: |
Qin YQ; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Liu W; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Yin R; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Xiao PT; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Wang ZY; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Huang TQ; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China., Liu EH; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China. |
| Abstrakt: |
Two new natural products named 5,7-dihydroxy-3,3',6,8-tetramethoxy-4',5'-methylenedioxyflavone ( 1 ) and 3,3',5,7-tetramethoxy-4',5'-methylenedioxyflavone ( 2 ), along with thirteen known compounds, β-sitosterol ( 3 ), desmethoxyyangonin ( 4 ), hexadecane ( 5 ), 3,9-bis(2,4-di-tert-butylphenoxy)-2,4,8,10-tetraoxa-3,9-diphosphaspiro [5.5] undecane 3,9-dioxide ( 6 ), 2'6'-dihydroxy-4'-methoxydihydrochalcone ( 7 ), cardamonin ( 8 ), 3,3',5,6,7,8-hexamethoxy-4',5'-methylenedioxyflavone ( 9 ), isofraxidin ( 10 ), aniba dimer A ( 11 ), 3,3',4',5,5',8-hexamethoxy-6,7-methylenedioxyflavone ( 12 ), quercetin ( 13 ), quercitrin ( 14 ) and isoquercitrin ( 15 ) were isolated from Sarcandra glabra (Thunb.) Nakai by various chromatographic methods. Compounds 1 , 2 , 4 , 6 , 11 , and 12 were isolated from S. glabra for the first time. Their chemical structures were identified through the analysis of NMR and HR-MS spectra. The anti-inflammatory and cytotoxic activities of compounds 1-15 were evaluated in cell assays. The results indicated that compounds 1 , 7 , 8 , 10 , 14 , and 15 significantly inhibited the NO production in LPS-induced RAW 264.7 murine macrophage cells. Moreover, compounds 1 , 3 , 4 , 7 , 8 , 9 , 10 and 12 exhibited a cytotoxic effect on the human HepG2 cell line. |
