A Liquid Chromatography-Mass Spectrometry Method to Study the Interaction between Membrane Proteins and Low-Molecular-Weight Compound Mixtures.

Autor: Ogiso H; Toyama Prefectural Institute for Pharmaceutical Research, Imizu 939-0363, Toyama, Japan., Suno R; Department of Medical Chemistry, Kansai Medical University, Hirakata 573-1010, Osaka, Japan., Kobayashi T; Department of Medical Chemistry, Kansai Medical University, Hirakata 573-1010, Osaka, Japan., Kawami M; Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima City 734-8553, Hiroshima, Japan., Takano M; Department of Pharmaceutics and Therapeutics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima City 734-8553, Hiroshima, Japan., Ogasawara M; Toyama Prefectural Institute for Pharmaceutical Research, Imizu 939-0363, Toyama, Japan.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2022 Jul 30; Vol. 27 (15). Date of Electronic Publication: 2022 Jul 30.
DOI: 10.3390/molecules27154889
Abstrakt: Molecular interaction analysis is an essential technique for the study of biomolecular functions and the development of new drugs. Most current methods generally require manipulation to immobilize or label molecules, and require advance identification of at least one of the two molecules in the reaction. In this study, we succeeded in detecting the interaction of low-molecular-weight (LMW) compounds with a membrane protein mixture derived from cultured cells expressing target membrane proteins by using the size exclusion chromatography-mass spectrometry (SEC-MS) method under the condition of 0.001% lauryl maltose neopentyl glycol as detergent and atmospheric pressure chemical ionization. This method allowed us to analyze the interaction of a mixture of medicinal herbal ingredients with a mixture of membrane proteins to identify the two interacting ingredients. As it does not require specialized equipment (e.g., a two-dimensional liquid chromatography system), this SEC-MS method enables the analysis of interactions between LMW compounds and relatively high-expressed membrane proteins without immobilization or derivatization of the molecules.
Databáze: MEDLINE
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