Autor: |
Avilés-Betanzos KA; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Subsede Sureste, Tablaje Catastral 31264, Km. 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de Yucatán, Sierra Papacal 97302, Mexico., Oney-Montalvo JE; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Subsede Sureste, Tablaje Catastral 31264, Km. 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de Yucatán, Sierra Papacal 97302, Mexico., Cauich-Rodríguez JV; Centro de Investigación Científica de Yucatán, Unidad de Materiales, Calle 43 No. 130 x 32 y 34, Colonia Chuburná de Hidalgo, Mérida 97205, Mexico., González-Ávila M; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Ex vivo Digestion Laboratory, CIATEJ, Normalistas No. 800, Colinas de la Normal 44270, Mexico., Scampicchio M; Faculty of Science and Technology, Free University of Bolzen-Bolzano, Piazza Università 1, 39100 Bolzano, Italy., Morozova K; Faculty of Science and Technology, Free University of Bolzen-Bolzano, Piazza Università 1, 39100 Bolzano, Italy., Ramírez-Sucre MO; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Subsede Sureste, Tablaje Catastral 31264, Km. 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de Yucatán, Sierra Papacal 97302, Mexico., Rodríguez-Buenfil IM; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Subsede Sureste, Tablaje Catastral 31264, Km. 5.5 Carretera Sierra Papacal-Chuburná Puerto, Parque Científico Tecnológico de Yucatán, Sierra Papacal 97302, Mexico. |
Abstrakt: |
Habanero pepper leaves and stems (by-products) have been traditionally considered waste; however, bioactive compounds such as polyphenols, vitamin C and carotenoids have been identified that can be used for formulation of nutraceuticals or functional foods. Furthermore, the extraction of these bioactive compounds by using environmentally friendly methods and solvents is desirable. Thus, the aim of this study was to assess the antioxidant capacity, total polyphenol content (TPC), the phenolic profile and vitamin C content in extracts obtained from by-products (stems and leaves) of two varieties (Mayapan and Jaguar) of habanero pepper by ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES). The results showed that NADES leads to extracts with significantly higher TPC, higher concentrations of individual polyphenols (gallic acid, protocatechuic acid, chlorogenic acid, cinnamic acid, coumaric acid), vitamin C and, finally, higher antioxidant capacity (9.55 ± 0.02 eq mg Trolox/g DM) than UAE extraction performed with methanol as the solvent. The association of individual polyphenols with NADES was confirmed by principal component analysis (PCA). Overall, NADES is an innovative and promising "green" extraction technique that can be applied successfully for the extraction of phenolic compounds from habanero pepper by-products. |