| Autor: |
Mantile F; Institute of Genetics and Biophysics (IGB) 'Adriano Buzzati Traverso', National Research Council (CNR) of Italy, 80131 Naples, Italy., Kisovec M; Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia., Adamo G; Institute for Research and Biomedical Innovation (IRIB), CNR, 90146 Palermo, Italy., Romancino DP; Institute for Research and Biomedical Innovation (IRIB), CNR, 90146 Palermo, Italy., Hočevar M; Department of Physics and Chemistry of Materials, Institute of Metals and Technology, SI-1000 Ljubljana, Slovenia., Božič D; Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.; Faculty of Electrical Engineering, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Bedina Zavec A; Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia., Podobnik M; Department of Molecular Biology and Nanobiotechnology, National Institute of Chemistry, SI-1000 Ljubljana, Slovenia., Stoppelli MP; Institute of Genetics and Biophysics (IGB) 'Adriano Buzzati Traverso', National Research Council (CNR) of Italy, 80131 Naples, Italy., Kisslinger A; Institute of Experimental Endocrinology and Oncology (IEOS), National Research Council (CNR) of Italy, 80131 Naples, Italy., Bongiovanni A; Institute for Research and Biomedical Innovation (IRIB), CNR, 90146 Palermo, Italy., Kralj-Iglič V; Faculty of Health Sciences, University of Ljubljana, SI-1000 Ljubljana, Slovenia.; Faculty of Electrical Engineering, University of Ljubljana, SI-1000 Ljubljana, Slovenia., Liguori GL; Institute of Genetics and Biophysics (IGB) 'Adriano Buzzati Traverso', National Research Council (CNR) of Italy, 80131 Naples, Italy. |
| Abstrakt: |
Tumor growth and metastasis strongly rely on cell-cell communication. One of the mechanisms by which tumor cells communicate involves the release and uptake of lipid membrane encapsulated particles full of bioactive molecules, called extracellular vesicles (EVs). EV exchange between cancer cells may induce phenotype changes in the recipient cells. Our work investigated the effect of EVs released by teratocarcinoma cells on glioblastoma (GBM) cells. EVs were isolated by differential centrifugation and analyzed through Western blot, nanoparticle tracking analysis, and electron microscopy. The effect of large EVs on GBM cells was tested through cell migration, proliferation, and drug-sensitivity assays, and resulted in a specific impairment in cell migration with no effects on proliferation and drug-sensitivity. Noticeably, we found the presence of the EGF-CFC founder member CRIPTO on both small and large EVs, in the latter case implicated in the EV-mediated negative regulation of GBM cell migration. Our data let us propose a novel route and function for CRIPTO during tumorigenesis, highlighting a complex scenario regulating its effect, and paving the way to novel strategies to control cell migration, to ultimately improve the prognosis and quality of life of GBM patients. |
