Exploring in vitro anti-proliferative and anti-inflammatory activities of Prasachandaeng remedy, and its bioactive compounds.
| Autor: | Prommee N; Department of Applied Thai Traditional Medicine and Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Faculty of Medicine, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand., Itharat A; Department of Applied Thai Traditional Medicine and Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Faculty of Medicine, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand. iarunporn@yahoo.com., Thongdeeying P; Department of Applied Thai Traditional Medicine and Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Faculty of Medicine, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand., Makchuchit S; Department of Applied Thai Traditional Medicine and Center of Excellence in Applied Thai Traditional Medicine Research (CEATMR), Faculty of Medicine, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand., Pipatrattanaseree W; Region Medicine Science Center 12 Songkhla, Department of Medical Science, Songkhla, 90110, Thailand., Tasanarong A; Chulabhorn International College of Medicine, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand.; Nephrology Unit, Faculty of Medicine, Thammasat University, Thammasat University (Rangsit Campus), Klong Luang, Pathum Thani, 12120, Thailand., Ooraikul B; Department of Agricultural Food and Nutritional Science, Faculty of Agricultural Life and Environmental Sciences, University of Alberta, Edmonton, AB, T6G2E1, Canada.; Bua Luang ASEAN, Thammasat University, Pathum Thani, 12120, Thailand., Davies NM; Faculty of Pharmacy and Pharmaceutical Sciences, Katz Centre for Pharmacy and Health Research, University of Alberta, Edmonton, AB, T6G2E1, Canada. |
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| Jazyk: | angličtina |
| Zdroj: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2022 Aug 11; Vol. 22 (1), pp. 217. Date of Electronic Publication: 2022 Aug 11. |
| DOI: | 10.1186/s12906-022-03678-y |
| Abstrakt: | Background: Prasachandaeng (PSD) remedy has been empirically used in Thai traditional medicine to treat fever in bile duct and liver and cancer patients through Thai folk doctors. However, there have been no scientific reports on the bioactive compounds and bioactivities related to inflammation-associated carcinogenesis or cytotoxicity against cancer cell lines. In this study, we investigated the chemical content of the remedy, and evaluated its cytotoxic activity against two cancer cell lines in comparison with a non-cancerous cell line and determined tumor necrosis factor-alpha (TNF-α) production in a murine macrophage cell line (RAW 264.7) to evaluate anti-inflammatory activity. A novel HPLC method was used for quality control of its chemical content. Methods: Pure compounds from the EtOH extract of D. cochinchinensis were isolated using bioassay-guided fractionation and chemical content of the PSD remedy was determined using HPLC. The cytotoxic activity against the hepatocarcinoma cell line (HepG2) and cholangiocarcinoma cell line (KKU-M156), in comparison with non-cancerous cell line (HaCaT), were investigated using antiproliferative assay (SRB). The anti-inflammatory activity measured by TNF-α production in RAW 264.7 was determined using ELISA. Results: All crude extracts and isolated compounds exhibited significant differences from vincristine sulfate ( **** p < 0.0001) in their cytotoxic activity against HepG2, KKU-M156, and HaCaT. The PSD remedy exhibited cytotoxic activity against HepG2 and KKU-M156 with IC Conclusion: The findings provide evidence of bioactivity for EtOH extracts of PSD remedy and the isolated compounds of D. Cochinchinensis. The results consistent the use clinical activity and use of PSD remedy as a antipyretic treatment for liver and bile duct cancer patients by Thai traditional practitioners. (© 2022. The Author(s).) |
| Databáze: | MEDLINE |
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